D'Ugo Emilio, Bertuccini Lucia, Spadaro Francesca, Giuseppetti Roberto, Iosi Francesca, Santavenere Fabio, Giuliani Fausto, Bruno Milena, Lovecchio Nicola, Gioacchini Silvia, Bucci Paola, Stellacci Emilia, Bernardo Antonietta, Mukherjee Arghya, Magurano Fabio
Department of Infectious Diseases, Istituto Superiore di Sanità, Rome, Italy.
Core Facilities, Istituto Superiore di Sanità, Rome, Italy.
Data Brief. 2022 Jul 11;43:108447. doi: 10.1016/j.dib.2022.108447. eCollection 2022 Aug.
Biofilm at water-oil interface of hypoxic water columns of microcosms, prepared from a lacustrine sample, that used diesel as a carbon source was found to show electrogenic properties. These microcosms named, Liquid Microbial Fuel Cells (L-MFCs) were electrically characterized using a custom electronic analyzer; accurate determination of voltage (V), power density (W/m 2), and current density (A/m2) for both charge and discharge phases was carried out. The instrument made it possible to carry out cell characterizations using resistive loads between 0 Ω (Ohm) and 10 kΩ. During the hypoxic and electrogenic phase, the synthesis of a system of "bacterial piping induction", produced filaments of hundreds of micrometers in which the microbial cells are hosted. Ultrastructural microscopy collected by scanning (SEM), transmission (TEM), immunofluorescence, Thunder Imager 3D, confocal laser scanning (CLSM) microscopy revealed a "myelin like" structure during filamentation processes; this "myelin like" structure exhibited cross-reactivity towards different epitopes of the myelin basic protein (MBP) and Claudin 11 (O4) of human oligodendrocytes. The disclosure of these filamentation processes could be helpful to describe further unconventional microbial structures in aquatic ecosystems and of the animal world. The data that support the findings of this study are openly available in at https://data.mendeley.com/datasets/7d35tj3j96/1.
从一个以柴油为碳源的湖相样本制备的微观世界缺氧水柱的水 - 油界面处发现的生物膜显示出产电特性。这些被命名为液体微生物燃料电池（L - MFCs）的微观世界使用定制电子分析仪进行电学表征；对充电和放电阶段的电压（V）、功率密度（W/m²）和电流密度（A/m²）进行了精确测定。该仪器使得使用0Ω（欧姆）至10kΩ之间的电阻负载进行电池表征成为可能。在缺氧和产电阶段，“细菌管道诱导”系统的合成产生了数百微米长的细丝，微生物细胞存在于其中。通过扫描电子显微镜（SEM）、透射电子显微镜（TEM）、免疫荧光、雷霆成像3D、共聚焦激光扫描显微镜（CLSM）收集的超微结构显微镜显示，在丝状化过程中存在一种“髓磷脂样”结构；这种“髓磷脂样”结构对人少突胶质细胞的髓磷脂碱性蛋白（MBP）和Claudin 11（O4）的不同表位表现出交叉反应性。这些丝状化过程的揭示可能有助于进一步描述水生生态系统和动物世界中非常规的微生物结构。支持本研究结果的数据可在https://data.mendeley.com/datasets/7d35tj3j96/1上公开获取。
