Zhang Chenguang, Zhang XIaoshuang, Cheng Bo, Wu Junkai, Zhang Libin, Xiao Xiao, Zhang Dong, Zhao Caiping, An Na, Han Mingyu, Xing Libo
Hebei Key Laboratory of Horticultural Germplasm Excavation and Innovative Utilization, College of Horticulture Technology, Hebei Normal University of Science and Technology, Changli, China.
College of Horticulture, Northwest A&F University, Yangling, China.
Front Plant Sci. 2022 Jul 5;13:903808. doi: 10.3389/fpls.2022.903808. eCollection 2022.
Flowering-related problems in "Fuji" apple have severely restricted the development of China's apple industry. Nuclear pore complexes (NPCs) control nucleoplasmic transport and play an important role in the regulation of plant growth and development. However, the effects of NPCs on apple flowering have not been reported. Here, we analysed the expression and function of , a component of apple NPC. expression was the highest in flower buds and maintained during 30-70 days after flowering. -overexpressing (OE) lines displayed significantly earlier flowering than that of the wild type. We further confirmed that MdNup54 interacts with MdHSP70, MdMYB11, and MdKNAT4/6. Consistent with these observations, flowering time of -OE lines was also significantly earlier. Therefore, our findings suggest a possible interaction of MdNup54 with MdHSP70 to mediate its nuclear and cytoplasmic transport and to regulate apple flowering. The results enhance the understanding of the flowering mechanism in apple and propose a novel strategy to study nucleoporins.
“富士”苹果中与开花相关的问题严重制约了中国苹果产业的发展。核孔复合体（NPCs）控制核质运输，并在植物生长发育的调控中发挥重要作用。然而，NPCs对苹果开花的影响尚未见报道。在此，我们分析了苹果NPC的一个组分 的表达和功能。 在花芽中表达最高，并在开花后30 - 70天保持。 过表达（OE）系的开花时间明显早于野生型。我们进一步证实MdNup54与MdHSP70、MdMYB11和MdKNAT4/6相互作用。与这些观察结果一致， -OE系的开花时间也明显更早。因此，我们的研究结果表明MdNup54与MdHSP70可能存在相互作用，以介导其核质运输并调节苹果开花。这些结果加深了对苹果开花机制的理解，并提出了一种研究核孔蛋白的新策略。
