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MdNup62 与参与苹果开花和耐热性的 MdHSFs 的相互作用。

MdNup62 interactions with MdHSFs involved in flowering and heat-stress tolerance in apple.

机构信息

College of Horticulture, Northwest A&F University, 3 Taicheng Road, Yangling, 712100, Shaanxi, China.

出版信息

BMC Plant Biol. 2022 Jul 4;22(1):317. doi: 10.1186/s12870-022-03698-3.

DOI:10.1186/s12870-022-03698-3
PMID:35786201
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9251929/
Abstract

Because of global warming, the apple flowering period is occurring significantly earlier, increasing the probability and degree of freezing injury. Moreover, extreme hot weather has also seriously affected the development of apple industry. Nuclear pore complexes (NPCs) are main channels controlling nucleocytoplasmic transport, but their roles in regulating plant development and stress responses are still unknown. Here, we analysed the components of the apple NPC and found that MdNup62 interacts with MdNup54, forming the central NPC channel. MdNup62 was localized to the nuclear pore, and its expression was significantly up-regulated in 'Nagafu No. 2' tissue-cultured seedlings subjected to heat treatments. To determine MdNup62's function, we obtained MdNup62-overexpressed (OE) Arabidopsis and tomato lines that showed significantly reduced high-temperature resistance. Additionally, OE-MdNup62 Arabidopsis lines showed significantly earlier flowering compared with wild-type. Furthermore, we identified 62 putative MdNup62-interacting proteins and confirmed MdNup62 interactions with multiple MdHSFs. The OE-MdHSFA1d and OE-MdHSFA9b Arabidopsis lines also showed significantly earlier flowering phenotypes than wild-type, but had enhanced high-temperature resistance levels. Thus, MdNUP62 interacts with multiple MdHSFs during nucleocytoplasmic transport to regulate flowering and heat resistance in apple. The data provide a new theoretical reference for managing the impact of global warming on the apple industry.

摘要

由于全球变暖，苹果的花期明显提前，增加了受冻害的概率和程度。此外，极端炎热的天气也严重影响了苹果产业的发展。核孔复合物（NPCs）是控制核质转运的主要通道，但它们在调节植物发育和应激反应中的作用仍不清楚。在这里，我们分析了苹果 NPC 的组成部分，发现 MdNup62 与 MdNup54 相互作用，形成中央 NPC 通道。MdNup62 定位于核孔，其在“长富 2 号”组织培养幼苗受到热处理时的表达显著上调。为了确定 MdNup62 的功能，我们获得了 MdNup62 过表达（OE）的拟南芥和番茄系，这些系表现出明显的高温抗性降低。此外，OE-MdNup62 拟南芥系比野生型开花更早。此外，我们鉴定了 62 个可能的 MdNup62 相互作用蛋白，并证实了 MdNup62 与多个 MdHSFs 的相互作用。OE-MdHSFA1d 和 OE-MdHSFA9b 拟南芥系也比野生型表现出更早的开花表型，但具有增强的高温抗性水平。因此，MdNUP62 在核质转运过程中与多个 MdHSFs 相互作用，调节苹果的开花和耐热性。该数据为管理全球变暖对苹果产业的影响提供了新的理论参考。

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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c49a/9251929/1a45fcc0936c/12870_2022_3698_Fig3_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c49a/9251929/1af87207d8e4/12870_2022_3698_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c49a/9251929/99546f0782ca/12870_2022_3698_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c49a/9251929/9baf3dbca242/12870_2022_3698_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c49a/9251929/138ff19388d4/12870_2022_3698_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c49a/9251929/db608a63945a/12870_2022_3698_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c49a/9251929/33b089f7567e/12870_2022_3698_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c49a/9251929/d4f93ec2fe00/12870_2022_3698_Fig11_HTML.jpg

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