Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin, China.
School of Integrated Plant Science, Plant Biology Section, Cornell University, Ithaca, New York.
Plant Cell Environ. 2020 Jun;43(6):1452-1466. doi: 10.1111/pce.13741. Epub 2020 Feb 28.
Nuclear pore complexes (NPCs) are main channels controlling nucleocytoplasmic transport and are composed of approximately 30 nucleoporins (NUPs). Emerging evidence suggests that some NUP genes have specialized functions that challenge the traditional view of NPCs as structures of uniform composition. Here, we analysed the role of six outer-ring components of NPC at normal and warm growth temperatures by examining their loss-of-function mutants in Arabidopsis thaliana. All six NUP subunits, NUP85, NUP96, NUP 133, NUP 160, SEH1 and HOS1, have a non-redundant temperature-influenced function in one or more of the processes, including rosette growth, leaf architecture and intracellular immune receptor-mediated disease resistance. At the molecular level, NUP85 and NUP133 are required for mRNA export only at warm temperature and play a larger role in the localization of transcription factor at warm temperature. In addition, NUP96 and HOS1 are essential for the expression of high temperature-responsive genes, which is correlated with their larger activity in facilitating nuclear accumulation of the transcription factor PIF4 at warm temperature. Our results show that subunits of NPC have differential roles at different temperatures, suggesting the existence of temperature-influenced NPC complexes and activities.
核孔复合体（NPC）是控制核质转运的主要通道，由大约 30 种核孔蛋白（NUPs）组成。新出现的证据表明，一些 NUP 基因具有专门的功能，这挑战了 NPC 作为均匀组成结构的传统观点。在这里，我们通过在拟南芥中研究其功能丧失突变体，分析了 NPC 外环的六个组件在正常和温暖生长温度下的作用。所有六个 NUP 亚基，NUP85、NUP96、NUP133、NUP160、SEH1 和 HOS1，在一个或多个过程中具有非冗余的温度影响功能，包括罗勒生长、叶结构和细胞内免疫受体介导的抗病性。在分子水平上，NUP85 和 NUP133 仅在温暖温度下需要 mRNA 输出，并且在温暖温度下转录因子的定位中发挥更大的作用。此外，NUP96 和 HOS1 对于高温响应基因的表达是必需的，这与它们在温暖温度下促进转录因子 PIF4 核积累的更大活性相关。我们的结果表明，NPC 的亚基在不同温度下具有不同的作用，这表明存在受温度影响的 NPC 复合物和活性。
