Akhtari Arezou, Davari Mahdi, Habibi-Yangjeh Aziz, Ebadollahi Asgar, Feizpour Solmaz
Department of Plant Protection, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran.
Department of Chemistry, Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran.
Front Plant Sci. 2022 Jul 5;13:932475. doi: 10.3389/fpls.2022.932475. eCollection 2022.
The utilization of plant essential oils (EOs) and nanomaterials due to their safety compared with synthetic chemicals has been considered in the management of plant diseases. In this study, the inhibitory effects of , , , and EOs in pure and Zinc Oxide (ZnO) nanocapsulated formulations were evaluated on the mycelial growth of to find a suitable alternative for synthetic chemicals. The crystal structure and morphological properties of the fabricated nanomaterials were assessed X-ray diffraction (XRD) and scanning electron microscope (SEM) analyses. The textural features of the prepared nanoparticles were investigated with Brunauer-Emmett-Teller (BET) analysis, and the presence of elements in the samples was studied with energy-dispersive X-ray (EDX) technique. The mycelial growth inhibitory (MGI) was performed in the laboratory by mixing with potato dextrose agar (PDA) medium at concentrations of 100, 300, 600, 1,000, 1,500, and 2,000 ppm. Based on the results, major differences were monitored between different concentrations. At the highest studied concentration, the inhibition of EO was 100%, which was 43.20, 42.37, and 21.19% for , , and , respectively, and the inhibition of their nanocapsules was 100, 51.32, 55.23, and 26.58%, respectively. In the greenhouse study, EO and its nanocapsule (ZnO-ZmEO) were compared with the ZnO and chlorothalonil fungicide based on the highest inhibitory of . The highest antifungal effect was related to the ZnO-ZmEO by 53.33%. Therefore, the ZnO-ZmEO formulation can be recommended as a biofungicide for managing and controlling tomato early blight disease after further research.
与合成化学品相比，植物精油（EOs）和纳米材料因其安全性而被考虑用于植物病害管理。在本研究中，评估了纯的以及氧化锌（ZnO）纳米胶囊化制剂中的[具体几种精油名称未给出]精油对[某种病原菌名称未给出]菌丝生长的抑制作用，以寻找合成化学品的合适替代品。通过X射线衍射（XRD）和扫描电子显微镜（SEM）分析评估了制备的纳米材料的晶体结构和形态特性。用布鲁诺尔-埃米特-泰勒（BET）分析研究了制备的纳米颗粒的纹理特征，并用能量色散X射线（EDX）技术研究了样品中元素的存在情况。通过在实验室中将[精油名称未给出]与马铃薯葡萄糖琼脂（PDA）培养基以100、300、600、1000、1500和2000 ppm的浓度混合来进行菌丝生长抑制（MGI）试验。根据结果，监测到不同浓度之间存在主要差异。在研究的最高浓度下，[某种精油名称未给出]精油的抑制率为100%，[其他几种精油名称未给出]的抑制率分别为43.20%、42.37%和21.19%，它们的纳米胶囊的抑制率分别为100%、51.32%、55.23%和26.58%。在温室研究中，基于[某种精油名称未给出]的最高抑制率，将[该精油名称未给出]精油及其纳米胶囊（ZnO-ZmEO）与ZnO和百菌清杀菌剂进行了比较。最高的抗真菌效果与ZnO-ZmEO相关，为53.33%。因此，经过进一步研究后，ZnO-ZmEO制剂可被推荐作为一种生物杀菌剂用于防治番茄早疫病。
