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分泌型白细胞蛋白酶抑制剂对大鼠模型前交叉韧带重建术后早期腱骨愈合的影响。

Effect of secretory leucocyte protease inhibitor on early tendon-to-bone healing after anterior cruciate ligament reconstruction in a rat model.

作者信息

Wu Yongmao, Shao Yan, Xie Denghui, Pan Jianying, Chen Huabin, Yao Juncheng, Liang Jiarong, Ke Haolin, Cai Daozhang, Zeng Chun

机构信息

Department of Orthopedics, Orthopedic Hospital of Guangdong Province, Academy of Orthopedics, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.

The Third School of Clinical Medicine, Southern Medical University, Guangzhou, China.

出版信息

Bone Joint Res. 2022 Jul;11(7):503-512. doi: 10.1302/2046-3758.117.BJR-2021-0358.R2.

DOI:10.1302/2046-3758.117.BJR-2021-0358.R2
PMID:35866455
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9350708/
Abstract

AIMS

To verify whether secretory leucocyte protease inhibitor (SLPI) can promote early tendon-to-bone healing after anterior cruciate ligament (ACL) reconstruction.

METHODS

In vitro: the mobility of the rat bone mesenchymal stem cells (BMSCs) treated with SLPI was evaluated by scratch assay. Then the expression levels of osteogenic differentiation-related genes were analyzed by real-time quantitative PCR (qPCR) to determine the osteogenic effect of SLPI on BMSCs. In vivo: a rat model of ACL reconstruction was used to verify the effect of SLPI on tendon-to-bone healing. All the animals of the SLPI group and the negative control (NC) group were euthanized for histological evaluation, micro-CT scanning, and biomechanical testing.

RESULTS

SLPI improved the migration ability of BMSCs and upregulated the expression of genes related to osteogenic differentiation of BMSCs in vitro. In vivo, the SLPI group had higher histological scores at the tendon-bone interface by histological evaluation. Micro-CT showed more new bone formation and bone ingrowth around the grafted tendon in the SLPI group. Evaluation of the healing strength of the tendon-bone connection showed that the SLPI group had a higher maximum failure force and stiffness.

CONCLUSION

SLPI can effectively promote early tendon-to-bone healing after ACL reconstruction via enhancing the migration and osteogenic differentiation of BMSCs. Cite this article:  2022;11(7):503-512.

摘要

目的

验证分泌型白细胞蛋白酶抑制剂（SLPI）能否促进前交叉韧带（ACL）重建术后早期腱骨愈合。

方法

体外实验：采用划痕实验评估经SLPI处理的大鼠骨髓间充质干细胞（BMSCs）的迁移能力。然后通过实时定量聚合酶链反应（qPCR）分析成骨分化相关基因的表达水平，以确定SLPI对BMSCs的成骨作用。体内实验：采用大鼠ACL重建模型验证SLPI对腱骨愈合的影响。对SLPI组和阴性对照组的所有动物实施安乐死，进行组织学评估、显微CT扫描和生物力学测试。

结果

体外实验中，SLPI提高了BMSCs的迁移能力，并上调了BMSCs成骨分化相关基因的表达。体内实验中，组织学评估显示SLPI组在腱骨界面处具有更高的组织学评分。显微CT显示SLPI组移植肌腱周围有更多新骨形成和骨长入。腱骨连接愈合强度评估显示，SLPI组具有更高的最大破坏力和刚度。

结论

SLPI可通过增强BMSCs的迁移和成骨分化，有效促进ACL重建术后早期腱骨愈合。引用本文：2022；11（7）：503-512。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fdd/9350708/66da02bce117/BJR-11-503-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fdd/9350708/7a641aead861/BJR-11-503-g0001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fdd/9350708/cd6e50895b9b/BJR-11-503-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fdd/9350708/66da02bce117/BJR-11-503-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fdd/9350708/7a641aead861/BJR-11-503-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fdd/9350708/2426047b6927/BJR-11-503-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fdd/9350708/3ac2c77cb233/BJR-11-503-g0003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fdd/9350708/6e9872b99158/BJR-11-503-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fdd/9350708/0f8155719c4e/BJR-11-503-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fdd/9350708/cd6e50895b9b/BJR-11-503-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fdd/9350708/66da02bce117/BJR-11-503-g0008.jpg

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