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用于斑马鱼条件性和跨神经元基因表达的病毒工具包。

A viral toolbox for conditional and transneuronal gene expression in zebrafish.

机构信息

Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.

Gene Delivery Technology Core, Massachusetts General Hospital, Cambridge, United States.

出版信息

Elife. 2022 Jul 22;11:e77153. doi: 10.7554/eLife.77153.

DOI:10.7554/eLife.77153
PMID:35866706
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9307271/
Abstract

The zebrafish is an important model in systems neuroscience but viral tools to dissect the structure and function of neuronal circuitry are not established. We developed methods for efficient gene transfer and retrograde tracing in adult and larval zebrafish by herpes simplex viruses (HSV1). HSV1 was combined with the Gal4/UAS system to target cell types with high spatial, temporal, and molecular specificity. We also established methods for efficient transneuronal tracing by modified rabies viruses in zebrafish. We demonstrate that HSV1 and rabies viruses can be used to visualize and manipulate genetically or anatomically identified neurons within and across different brain areas of adult and larval zebrafish. An expandable library of viruses is provided to express fluorescent proteins, calcium indicators, optogenetic probes, toxins and other molecular tools. This toolbox creates new opportunities to interrogate neuronal circuits in zebrafish through combinations of genetic and viral approaches.

摘要

斑马鱼是系统神经科学中的重要模型，但用于剖析神经元回路的结构和功能的病毒工具尚未建立。我们开发了通过单纯疱疹病毒（HSV1）在成年和幼鱼斑马鱼中进行高效基因转移和逆行示踪的方法。HSV1 与 Gal4/UAS 系统相结合，以具有高空间、时间和分子特异性的方式靶向细胞类型。我们还建立了在斑马鱼中通过改良的狂犬病病毒进行高效跨神经元示踪的方法。我们证明 HSV1 和狂犬病病毒可用于可视化和操纵成年和幼鱼不同脑区中经基因或解剖学鉴定的神经元。提供了一个可扩展的病毒文库，用于表达荧光蛋白、钙指示剂、光遗传学探针、毒素和其他分子工具。该工具包通过遗传和病毒方法的组合，为研究斑马鱼中的神经元回路创造了新的机会。

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The Dorsal Lateral Habenula-Interpeduncular Nucleus Pathway Is Essential for Left-Right-Dependent Decision Making in Zebrafish.
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