College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, China.
J Food Biochem. 2022 Oct;46(10):e14323. doi: 10.1111/jfbc.14323. Epub 2022 Jul 22.
A novel polysaccharide was extracted from Coriandrum sativum L. at a yield of 4.56 ± 0.17% (n = 3). The extraction was optimized using response surface methodology: powder-to-liquid ratio 1:21 g/ml, extraction time 188 min, temperature 81°C, and three replicate extractions. The purified polysaccharide had an average molecular weight of 1.30 × 10 Da and was composed of rhamnose, arabinose, galactose, glucose, and galacturonic acid in molar ratios of 1.52: 8.14: 20.85: 1: 2.42 with α-L-Araf-(1→, →6)-β-D-Galp-(1→, →4)-α-GalpA-(1→ and →2, 4)-α-Rhap-(1→). In vivo tests demonstrated that the polysaccharide suppressed H22 tumor growth in mice and protected the immune organs. Annexin V-FITC/PI, PI, and JC-1 staining showed that the primary mechanism of tumor inhibition was the induction of apoptosis and S-phase arrest with apoptosis achieved via a mitochondrial pathway. PRACTICAL APPLICATIONS: Coriandrum sativum L. is used as a culinary spice but its medicinal value has also been widely recognized. A novel polysaccharide was extracted from this herbaceous plant and its structure and bioactivity were investigated. This high-molecular-weight polysaccharide exhibited antitumor effects against H22 cells in mice and had potential to be developed as an anti-liver cancer medicine and functional food supplement.
从芫荽中提取出一种新型多糖，产率为 4.56±0.17%（n=3）。采用响应面法对提取条件进行优化：料液比 1:21 g/ml、提取时间 188 min、温度 81°C，重复提取 3 次。纯化后的多糖平均分子量为 1.30×10 Da，由鼠李糖、阿拉伯糖、半乳糖、葡萄糖和半乳糖醛酸以摩尔比 1.52:8.14:20.85:1:2.42组成，其结构为α-L-Araf-(1→, →6)-β-D-Galp-(1→, →4)-α-GalpA-(1→和→2, 4)-α-Rhap-(1→。体内试验表明，该多糖能抑制小鼠 H22 肿瘤生长，保护免疫器官。Annexin V-FITC/PI、PI 和 JC-1 染色表明，肿瘤抑制的主要机制是诱导细胞凋亡和 S 期阻滞，凋亡是通过线粒体途径实现的。实际应用：芫荽既是一种烹饪香料，其药用价值也得到了广泛认可。从这种草本植物中提取出一种新型多糖，并对其结构和生物活性进行了研究。这种高分子量多糖对小鼠 H22 细胞具有抗肿瘤作用，有望开发为抗肝癌药物和功能性食品补充剂。
