种子多糖的结构表征：对体内镉毒性的肝保护作用

Structural Characterization of Polysaccharides from Seeds: Hepatoprotective Effect against Cadmium Toxicity In Vivo.

作者信息

Sfar Manel, Souid Ghada, Alminderej Fahad M, Mzoughi Zeineb, El-Ghoul Yassine, Rihouey Christophe, Le Cerf Didier, Majdoub Hatem

机构信息

Laboratory of Interfaces and Advanced Materials, Faculty of Sciences of Monastir, University of Monastir, Monastir 5000, Tunisia.

Research Unit: Viral Genomic and Antiviral Strategy (UR 17ES 30), University of Monastir, Higher Institute of Biotechnology of Monastir, Monastir 5000, Tunisia.

出版信息

Antioxidants (Basel). 2023 Feb 10;12(2):455. doi: 10.3390/antiox12020455.

DOI:10.3390/antiox12020455

PMID:36830010

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9952120/

Abstract

is one of the most widespread curative plants in the world, being vastly cultivated in arid and semi-arid regions as one of the oldest spice plants. The present study explored the extraction of polysaccharides from Coriandrum sativum seeds and the evaluation of their antioxidant potential and hepatoprotective effects in vivo. The polysaccharide from coriander seeds was extracted, and the structural characterization was performed by FT-IR, UV-vis, DSC, NMR (1D and 2D), GC-MS, and SEC analysis. The polysaccharide extracted from (CPS) seeds was characterized to evaluate its antioxidant and hepatoprotective capacities in rats. Results showed that CPS was composed of arabinose, rhamnose, xylose, mannose, fructose, galactose, and glucose in molar percentages of 6.2%, 3.6%, 8.8%, 17.7%, 5.2%, 32.9%, and 25.6%, respectively. Further, CPS significantly hindered cadmium-induced oxidation damage and exercised a protective effect against Cd hepatocytotoxicity, with a considerable reduction in MDA production and interesting CAT and SOD enzyme levels. Results suggest that CPS might be employed as a natural antioxidant source.

摘要

是世界上分布最广的药用植物之一，作为最古老的香料植物之一，在干旱和半干旱地区广泛种植。本研究探讨了从芫荽籽中提取多糖及其体内抗氧化潜力和肝脏保护作用的评估。提取了芫荽籽中的多糖，并通过傅里叶变换红外光谱（FT-IR）、紫外可见光谱（UV-vis）、差示扫描量热法（DSC）、核磁共振（NMR，一维和二维）、气相色谱-质谱联用（GC-MS）和尺寸排阻色谱（SEC）分析进行了结构表征。对从（CPS）种子中提取的多糖进行表征，以评估其在大鼠体内的抗氧化和肝脏保护能力。结果表明，CPS由阿拉伯糖、鼠李糖、木糖、甘露糖、果糖、半乳糖和葡萄糖组成，摩尔百分比分别为6.2%、3.6%、8.8%、17.7%、5.2%、32.9%和25.6%。此外，CPS显著抑制了镉诱导的氧化损伤，并对镉肝细胞毒性发挥了保护作用，丙二醛（MDA）生成量显著降低，过氧化氢酶（CAT）和超氧化物歧化酶（SOD）酶水平也呈现出有趣的变化。结果表明，CPS可能被用作天然抗氧化剂来源。