Radiation Biology Research Center, Iran University of Medical Sciences, Tehran, Iran.
Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran.
Photobiomodul Photomed Laser Surg. 2022 Jul;40(7):488-498. doi: 10.1089/photob.2022.0012.
Spinal cord injury (SCI) causes motor deficits, urinary incontinence, and neuropathic pain. This study was designed to optimize a photobiomodulation therapy (PBMT) protocol using a continuous wave (CW) 660 nm laser in rats with SCI. Specifically, the number of days of irradiation and the daily dose of PBMT were investigated. The study was performed in two steps. In the first step, a comparison between the effects of PBMT (45 sec) daily for 2 and 4 weeks on pain and movement [Basso, Beattie, and Brenham (BBB) score] was made. In the second step, a comparison between different durations of irradiation (27, 45, 90, and 117 sec) was performed. PBMT used a 100 mW laser delivered to 9 points on and around the lesion site. Oxidative stress, fibroblast invasion, and time to achieve spontaneous urination were also assessed. The improvement in movement and pain stopped with discontinuation of radiation at week 2 and fibroblast invasion resumed. No improvement was seen in movement and pain in the group receiving PBMT for 27 sec compared with the groups receiving higher doses of laser radiation. Animals receiving 117 sec of photobiomodulation showed a higher BBB score even in the first 3 days. The number of days is an important factor for improving mobility; however, the daily dose of radiation is more important for pain relief.
脊髓损伤 (SCI) 会导致运动功能障碍、尿失禁和神经性疼痛。本研究旨在通过使用连续波 (CW) 660nm 激光优化 SCI 大鼠的光生物调节疗法 (PBMT) 方案。具体来说，研究了辐照天数和 PBMT 日剂量。该研究分两步进行。在第一步中，比较了每天照射 45 秒，持续 2 周和 4 周对疼痛和运动（Basso、Beattie 和 Brenham [BBB] 评分）的影响。在第二步中，比较了不同的辐照时间（27、45、90 和 117 秒）。PBMT 使用 100mW 激光照射损伤部位及其周围的 9 个点。还评估了氧化应激、成纤维细胞浸润和实现自发性排尿的时间。运动和疼痛的改善在第 2 周停止照射后停止，成纤维细胞浸润恢复。与接受更高剂量激光辐射的组相比，接受 27 秒 PBMT 的组在运动和疼痛方面没有改善。接受 117 秒光生物调节的动物甚至在第 3 天的 BBB 评分也更高。照射天数是改善运动能力的一个重要因素；然而，辐射的日剂量对于缓解疼痛更为重要。
