Radiation Biology Research Center, Iran University of Medical Sciences, Tehran, Iran.
Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran.
Lasers Med Sci. 2023 May 9;38(1):120. doi: 10.1007/s10103-023-03722-4.
Neuropathic pain (NP) following spinal cord injury (SCI) often lasts for a long time and causes a range of problems that reduce the quality of life. Current treatments are not generally effective; however, photobiomodulation therapy (PBMT) has made some progress in this area. Due to the novelty of this treatment, standard therapeutic protocols have not yet been agreed upon. In the present study, we compare the analgesic effect of two PBMT protocols (2 and 4 weeks of radiation). A total of thirty-two adult male Wistar rats were divided into four groups: control, SCI, 2 W PBMT, and 4 W PBMT. SCI was induced by an aneurism clip and PBMT used a 660-nm, initiated 30 min post-SCI, and continued daily for 2 or 4 weeks. Functional recovery, hyperalgesia, and allodynia were measured weekly. At the end of the study, the Gad65, interleukin 1-alpha (IL1α), interleukin 10 (IL10), IL4, and purinergic receptor (P2xR and P2yR) expressions were measured. Data were analyzed by Prism6. The results showed PBM irradiation for 2 and 4 weeks had the same effects in improving hyperalgesia. In the case of allodynia and functional recovery, 4 W PBMT was more effective (p<0.01). 4 W PBMT increased the Gad65 expression (p <0.001) and reduced P2Y4R (p <0.05) compared to SCI animals. The effects of 2 and 4 W PBMT were the same for IL1α, IL10, and P2X3 receptors. 4 W PBMT was more effective in reducing the complications of SCI such as pain and disability. PBMT therapy is an effective method aimed at immune system function modulation to reduce NP and motor dysfunction.
脊髓损伤（SCI）后常引起神经病理性疼痛（NP），持续时间长，并导致一系列降低生活质量的问题。目前的治疗方法通常效果不佳；然而，光生物调节疗法（PBMT）在这一领域取得了一些进展。由于这种治疗方法尚属新颖，尚未达成标准治疗方案。在本研究中，我们比较了两种 PBMT 方案（2 周和 4 周照射）的镇痛效果。总共 32 只成年雄性 Wistar 大鼠被分为 4 组：对照组、SCI 组、2 周 PBMT 组和 4 周 PBMT 组。SCI 通过动脉瘤夹诱导，PBMT 在 SCI 后 30 分钟开始使用 660nm 激光，每天照射，持续 2 或 4 周。每周测量功能恢复、痛觉过敏和感觉异常。研究结束时，测量 Gad65、白细胞介素 1-α（IL1α）、白细胞介素 10（IL10）、IL4 和嘌呤能受体（P2xR 和 P2yR）的表达。数据由 Prism6 分析。结果表明，PBMT 照射 2 周和 4 周对改善痛觉过敏具有相同的效果。在感觉异常和功能恢复方面，4 周 PBMT 更有效（p<0.01）。与 SCI 动物相比，4 周 PBMT 增加了 Gad65 的表达（p <0.001），降低了 P2Y4R（p <0.05）。2 周和 4 周 PBMT 对 IL1α、IL10 和 P2X3 受体的影响相同。4 周 PBMT 更有效地减少 SCI 引起的疼痛和残疾等并发症。PBMT 治疗是一种有效方法，旨在调节免疫系统功能，减轻 NP 和运动功能障碍。
