Department of Occupational and Environmental Health, School of Public Health, Wuhan University of Science and Technology, Wuhan, 430065, Hubei, China.
Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Wuhan University of Science and Technology, Wuhan, 430065, Hubei, China.
BMC Cancer. 2022 Jul 22;22(1):809. doi: 10.1186/s12885-022-09905-6.
Lung cancer is the leading cause of malignancy-related mortality and lung adenocarcinoma accounts for about 40% of lung malignancies. The aim of this study was to investigate the associations of intraflagellar transport protein 20 (IFT20) and Golgi matrix protein 130 (GM130) expression with clinicopathological features and survival in patients with lung adenocarcinoma.
The expressions of IFT20 and GM130 protein in cancerous and matched adjacent lung tissues of 235 patients with lung adenocarcinoma were assessed by tissue microarray and immunohistochemistry, which were indicated by the mean optical density (IOD/area), the rate of positive staining cells and staining intensity score. The correlation between IFT20 and GM130 protein was assessed by Spearman's rank correlation. Associations of IFT20 and GM130 protein expression with clinicopathological features of patients were analyzed by multivariate logistic regression models. The survival analysis of patients was performed by Cox proportional hazard regression models.
With adjustment for multiple potential confounders, each one-point increase in IFT20 protein staining intensity score was significantly associated with 32% and 29% reduced risk for TNM stage in II ~ IV and lymphatic metastasis of patients, respectively (P < 0.05). And each one-point increase in GM130 protein staining intensity score was associated with a significant reduction in the risk of poor differentiation and tumors size > 7 cm by 29% and 38% for lung adenocarcinoma patients, respectively (P < 0.05). In stratified Cox model analysis, enhanced IFT20 staining intensity score was significantly decreased the risk of death by 16% for patients without distant metastasis. And elevated the IOD/area of GM130 expression significantly decreased the death risk of lung adenocarcinoma patients with tumor size > 7 cm or distant metastasis by 54% and 65%, respectively (P < 0.05).
IFT20 and GM130 protein expressions were negatively associated with tumor differentiated types, size, TNM stage and lymphatic metastasis of lung adenocarcinoma. Both IFT20 and GM130 proteins have some protective effects on the survival of lung adenocarcinoma patients with specific clinicopathological features.
肺癌是恶性肿瘤相关死亡的主要原因，肺腺癌约占肺癌的 40%。本研究旨在探讨内纤毛转运蛋白 20（IFT20）和高尔基基质蛋白 130（GM130）表达与肺腺癌患者临床病理特征和生存的关系。
采用组织微阵列和免疫组织化学法检测 235 例肺腺癌患者癌组织及配对癌旁组织中 IFT20 和 GM130 蛋白的表达，以平均光密度（IOD/面积）、阳性染色细胞率和染色强度评分表示。采用 Spearman 秩相关分析评估 IFT20 和 GM130 蛋白之间的相关性。采用多因素 logistic 回归模型分析 IFT20 和 GM130 蛋白表达与患者临床病理特征的关系。采用 Cox 比例风险回归模型进行患者生存分析。
调整多个潜在混杂因素后，IFT20 蛋白染色强度评分每增加 1 分，患者 TNM 分期为 II~IV 期和淋巴转移的风险分别降低 32%和 29%（P<0.05）。GM130 蛋白染色强度评分每增加 1 分，肺腺癌患者低分化和肿瘤直径>7cm的风险分别降低 29%和 38%（P<0.05）。在分层 Cox 模型分析中，IFT20 染色强度评分升高可使无远处转移患者的死亡风险降低 16%。GM130 表达的 IOD/面积升高可使肿瘤直径>7cm 或有远处转移的肺腺癌患者的死亡风险分别降低 54%和 65%（P<0.05）。
IFT20 和 GM130 蛋白表达与肺腺癌的肿瘤分化类型、肿瘤大小、TNM 分期和淋巴转移呈负相关。IFT20 和 GM130 蛋白对具有特定临床病理特征的肺腺癌患者的生存有一定的保护作用。
