Laboratory of Molecular Neurobiology, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland.
Front Endocrinol (Lausanne). 2022 Jul 7;13:867929. doi: 10.3389/fendo.2022.867929. eCollection 2022.
Obesity is a growing medical and social problem worldwide. The control of energy homeostasis in the brain is achieved by various regions including the arcuate hypothalamic nucleus (ARH). The latter comprises a number of neuronal populations including the first order metabolic neurons, appetite-stimulating agouti-related peptide (AgRP) neurons and appetite-suppressing proopiomelanocortin (POMC) neurons. Using an reductionist approach and POMC-dependent CRISPR-Cas9, we demonstrate that miR-15a-5p protects from obesity. Moreover, we have identified , a gene previously linked to energy metabolism imbalance, as a direct target of miR-15a-5p. This work warrants further investigations of non-coding RNA-mediated regulation of energy homeostasis and might contribute to the development of novel therapeutic approaches to treat metabolic diseases.
肥胖是一个全球性的日益严重的医学和社会问题。大脑中能量平衡的控制是通过包括弓状下丘脑核（ARH）在内的各种区域来实现的。后者包含许多神经元群体，包括第一级代谢神经元、食欲刺激的 AgRP（Agouti 相关肽）神经元和食欲抑制的 POMC（Proopiomelanocortin）神经元。使用简化论方法和 POMC 依赖性 CRISPR-Cas9，我们证明 miR-15a-5p 可以预防肥胖。此外，我们已经确定了先前与能量代谢失衡相关的基因作为 miR-15a-5p 的直接靶标。这项工作需要进一步研究非编码 RNA 对能量平衡的调节作用，这可能有助于开发治疗代谢疾病的新的治疗方法。
