Chellapandi K, Subbarayan S, De Mandal S, Ralte L, Senthil Kumar N, Dutta T K, Sharma I
Dept. of Veterinary Microbiology, Central Agricultural University, Selesih, Aizawl, 796014, Mizoram, India.
Department of Microbiology, Assam University, Silchar, 788011, Assam, India.
New Microbes New Infect. 2022 Jun 30;48:100999. doi: 10.1016/j.nmni.2022.100999. eCollection 2022 Jul.
Antibiotic-resistance in is a global issue affecting humans especially the pediatric population. Antibiotic-resistant is a pathogen frequently pediatric population as well as healthy adults.
This study aimed to examine the antibiotic resistance of causing pediatric diarrhea and its drug-resistant rates, its adhering abilities to cell line in vitro, and inhibition efficiency of a few selected chemical compounds. Clinical strains were isolated from both the healthy and infected pediatric population of Mizoram.
Adhesion is a significant pathogenic process during bacterial infections, which has been employed for pathotyping of DEC by comparing adhesion efficiency in both normal (CHO-k1) and cancer (HeLa) cell lines. adherent pathotypes were identified by both PCR assay and in-vitro cell adhesion assays; the study also evaluated the adhesion inhibition ability of human skimmed milk, gentamicin, and cephalexin in-vitro. Of all isolates, 20.05% of adherent DEC (EPEC, DAEC, and EIEC) and 11.39 % of non-adherent DEC (STEC and ETEC) were found to be associated with pediatric diarrhoea in Mizoram. Human skimmed milk has a high potential adhesion inhibition against EAEC (50.25/90.90 μg/mL), EPEC (53.42/259.70 μg/mL), and EIEC (59.13/30.30 μg/mL) in both cell lines in comparison with gentamicin and cephalexin.
This study concludes that as a dietary supplement-human skimmed milk has high potential to prevent adhesion of DEC pathotypes in cells in-vitro thus in in-vivo.
[病原体名称]的抗生素耐药性是一个影响人类尤其是儿童群体的全球性问题。耐抗生素的[病原体名称]是一种常见于儿童群体以及健康成年人中的病原体。
本研究旨在检测引起儿童腹泻的[病原体名称]的抗生素耐药性及其耐药率、其在体外对细胞系的黏附能力以及几种选定化合物的抑制效率。临床菌株从米佐拉姆邦健康和感染的儿童群体中分离得到。
黏附是细菌感染过程中的一个重要致病过程，通过比较在正常（CHO - k1）和癌细胞（HeLa）系中的黏附效率，已被用于腹泻大肠埃希菌（DEC）的致病分型。通过聚合酶链反应（PCR）检测和体外细胞黏附试验鉴定出DEC黏附致病型；该研究还在体外评估了人脱脂牛奶、庆大霉素和头孢氨苄的黏附抑制能力。在所有分离株中，发现20.05%的黏附性DEC（肠致病性大肠埃希菌、弥漫性黏附性大肠埃希菌和肠侵袭性大肠埃希菌）和11.39%的非黏附性DEC（产志贺毒素大肠埃希菌和肠毒素性大肠埃希菌）与米佐拉姆邦的儿童腹泻有关。与庆大霉素和头孢氨苄相比，人脱脂牛奶在两种细胞系中对肠集聚性大肠埃希菌（50.25/90.90μg/mL）、肠致病性大肠埃希菌（53.42/259.70μg/mL）和肠侵袭性大肠埃希菌（59.13/30.30μg/mL）具有较高的潜在黏附抑制作用。
本研究得出结论，作为一种膳食补充剂，人脱脂牛奶在体外及体内均具有很高的潜力来预防DEC致病型在细胞中的黏附。
