Soro Monique, Tiendrébéogo Fidèle, Pita Justin S, Traoré Edwig T, Somé Koussao, Tibiri Ezechiel B, Néya James B, Mutuku J Musembi, Simporé Jacques, Koné Daouda
Central and West African Virus Epidemiology (WAVE) Pôle scientifique et d'innovation de Bingerville Université Félix Houphouët-Boigny (UFHB) Bingerville Ivory Coast.
Laboratoire de Biotechnologie, Agriculture et Valorisation des Ressources Biologiques UFR Biosciences Université Félix Houphouët-Boigny Abidjan Ivory Coast.
Plant Pathol. 2021 Dec;70(9):2207-2216. doi: 10.1111/ppa.13459. Epub 2021 Sep 9.
Surveys were conducted in 2016 and 2017 across the main cassava-growing regions of Burkina Faso to assess the status of cassava mosaic disease (CMD) and to determine the virus strains causing the disease, using field observation and phylogenetic analysis. CMD incidence varied between regions and across years but was lowest in Hauts-Bassins (6.0%, 2016 and 5.4%, 2017) and highest in Centre-Sud (18.5%, 2016) and in Boucle du Mouhoun (51.7%, 2017). The lowest CMD severity was found in Est region (2.0) for both years and the highest in Sud-Ouest region (3.3, 2016) and Centre-Sud region (2.8, 2017). The CMD infection was primarily associated with contaminated cuttings in all regions except in Hauts-Bassins, where whitefly-borne infection was higher than cuttings-borne infection in 2016. PCR screening of 687 samples coupled with sequence analysis revealed the presence of African cassava mosaic-like (ACMV-like) viruses and East African cassava mosaic-like (EACMV-like) viruses as single infections at 79.5% and 1.1%, respectively. Co-infections of ACMV-like and EACMV-like viruses were detected in 19.4% of the tested samples. In addition, 86.7% of the samples positive for EACMV-like virus were found to be positive for East African cassava mosaic Cameroon virus (EACMCMV). Phylogenetic analysis revealed the segregation of cassava mosaic geminiviruses (CMGs) from Burkina Faso into three clades specific to ACMV, African cassava mosaic Burkina Faso virus (ACMBFV), and EACMCMV, confirming the presence of these viruses. The results of this study show that EACMCMV occurrence may be more prevalent in Burkina Faso than previously thought.
2016年和2017年，在布基纳法索主要木薯种植区开展了调查，采用田间观察和系统发育分析来评估木薯花叶病（CMD）的状况，并确定引发该病的病毒株系。CMD发病率因地区和年份而异，在高原地区最低（2016年为6.0%，2017年为5.4%），在中南部地区最高（2016年为18.5%），在穆洪河环形地区最高（2017年为51.7%）。两年中，东部地区的CMD严重程度最低（2.0），而在西南部地区最高（2016年为3.3），在中南部地区（2017年为2.8）。除高原地区外，所有地区的CMD感染主要与受污染的插条有关，在高原地区，2016年粉虱传播的感染高于插条传播的感染。对687个样本进行PCR筛查并结合序列分析，结果显示，非洲木薯花叶样（ACMV样）病毒和东非木薯花叶样（EACMV样）病毒的单一感染率分别为79.5%和1.1%。在19.4%的测试样本中检测到ACMV样病毒和EACMV样病毒的共同感染。此外，在86.7%的EACMV样病毒呈阳性的样本中，还发现东非木薯花叶喀麦隆病毒（EACMCMV）呈阳性。系统发育分析表明，布基纳法索的木薯花叶双生病毒（CMG）分为三个进化枝，分别对应ACMV、非洲木薯花叶布基纳法索病毒（ACMBFV）和EACMCMV，证实了这些病毒的存在。本研究结果表明，EACMCMV在布基纳法索的出现可能比之前认为的更为普遍。
