Jiang Ying, Zhang Haiwen, Li Yang, Chang Congcong, Wang Yunxiao, Feng Hao, Li Ruifen
Agro-Biotechnology Research Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.
Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing, China.
Front Plant Sci. 2022 Jul 7;13:927253. doi: 10.3389/fpls.2022.927253. eCollection 2022.
Halophytic is a perennial grass which has evolved many distinctive salt-adaptive mechanisms. Our previous studies indicated it could thrive under salt stress through maintaining better K and Na homeostasis. Stress-responsive HbCIPK2 can phosphorylate K channel HbVGKC1 and Na transporter HbSOS1L to prevent Na accumulation and K reduction, hence pathway was not detected in glycophytic plants. In this study, we cloned the inducible promoter of by genome-walking, and identified a novel transcriptional regulator HbERF6 through yeast one-hybrid screening. HbERF6 functioned as a transcription factor which can bind to the GCC-box of the promoter to activate its expression. transgenic lines in improved salt tolerance compared with wild type, and especially induced () expression, resulting in K/Na homeostasis to enhance salt tolerance. All the results confirmed the inducible function of HbERF6 for CIPK genes during salt tolerance. This regulatory network that integrates transcriptional regulation and post-translation modification will unravel a novel salt stress-responsive mechanism, highlighting the value and utilization of the halophytic resource.
盐生植物是一种多年生草本植物，它进化出了许多独特的盐适应性机制。我们之前的研究表明，它可以通过维持更好的钾和钠稳态在盐胁迫下茁壮成长。应激反应性的HbCIPK2可以磷酸化钾通道HbVGKC1和钠转运体HbSOS1L，以防止钠积累和钾减少，因此在甜土植物中未检测到该途径。在本研究中，我们通过基因组步移克隆了 的诱导型启动子，并通过酵母单杂交筛选鉴定了一种新型转录调节因子HbERF6。HbERF6作为一种转录因子，可与 启动子的GCC框结合以激活其表达。与野生型相比， 中的转基因株系提高了耐盐性，尤其是诱导了 （）表达，从而实现钾/钠稳态以增强耐盐性。所有结果证实了HbERF6在耐盐过程中对CIPK基因的诱导功能。这种整合转录调控和翻译后修饰的调控网络将揭示一种新的盐胁迫响应机制，突出盐生植物资源的价值和利用。
