Abdel-Hameed Salwa A M, Ahmed Samia A, Mostafa Faten A, Almasarawi Ola N, Abdel Wahab Walaa A
Glass Research Department, National Research Centre, Dokki, Cairo, Egypt.
Chemistry of Natural and Microbial Products Department, National Research Centre, Dokki, Cairo, Egypt.
Heliyon. 2022 Jul 16;8(7):e09960. doi: 10.1016/j.heliyon.2022.e09960. eCollection 2022 Jul.
Bacterial α-amylase was immobilized on sugilite from modified basalt rock as a new carrier. A set of glass compositions based on sugilite formula KNaMLiSiO (M = Al or Mn or Fe) were prepared. The glasses were prepared through melting-quenching technique and samples of glass were converted to glass ceramic. Among the tested glasses and glass ceramic only sugilite glass based on M = Fe (BSF) give promising results. The sugilite BSF glass was characterized using DSC analysis, FTIR absorption, and SEM. The sugilite glass revealed high thermal resistant till ∼770 °C. Under optimized conditions of the Central composite design, the immobilization yield improved by 4.7-fold. The affinity to starch increased after enzyme immobilization by 4.3-fold. The lower rate of deactivation constant and the increase of and -value confirm the suitability of BSF and immobilization method in enhancing enzyme stability. The improvement in thermostability of immobilized α-amylase was judged by the change in thermodynamic parameters. In conclusion, the prepared sugilite BSF glass can be utilized as a new carrier suitable for stabilization of α-amylase enzyme by immobilization.
细菌α-淀粉酶被固定在改性玄武岩岩中的菅原矿上作为一种新型载体。制备了一组基于菅原矿化学式KNaMLiSiO（M = Al或Mn或Fe）的玻璃组合物。这些玻璃通过熔融淬火技术制备，玻璃样品转化为玻璃陶瓷。在测试的玻璃和玻璃陶瓷中，只有基于M = Fe的菅原矿玻璃（BSF）给出了有前景的结果。使用差示扫描量热法（DSC）分析、傅里叶变换红外吸收光谱（FTIR）和扫描电子显微镜（SEM）对菅原矿BSF玻璃进行了表征。菅原矿玻璃显示出高达约770°C的高热稳定性。在中心复合设计的优化条件下，固定化产率提高了4.7倍。酶固定化后对淀粉的亲和力增加了4.3倍。较低的失活常数速率以及 和 值的增加证实了BSF和固定化方法在提高酶稳定性方面的适用性。通过热力学参数的变化判断固定化α-淀粉酶热稳定性的提高。总之，制备的菅原矿BSF玻璃可作为一种新型载体，适用于通过固定化来稳定α-淀粉酶。
