Wang Jingru, Chen Yuanwen, He Jia, Li Guiqiang, Chen Xiaodong, Liu Hongwei
Department of Plastic Surgery, The First Affiliated Hospital of Jinan University, Guangzhou, China.
Department of Burn Surgery, First People's Hospital of Foshan, Foshan, China.
Front Surg. 2022 Jul 8;9:950967. doi: 10.3389/fsurg.2022.950967. eCollection 2022.
Adipose-derived stem cells(ADSCs) have been used for anti-photo-aging. But the purification of ADSCs requires amplification and culture, there is considerable risk of direct treatment for patients. Stromal vascular fraction(SVF) is a biologically and clinically interesting heterogeneous cell population contains ADSCs. There are few reports on anti-aging effects of SVF in photo-aging skin. The present study investigated the anti-aging effect of stromal vascular fraction (SVF) and adipose-derived stem cells (ADSCs) injection in photo-aging skin. The relationship between the dosage of injection and effect was also discussed. Thirty healthy, 6-week-old, nude rats were randomly divided into the control and experimental groups. The experimental group needing ultraviolet B (UVB) irradiation five days per week, and a duration of 8 weeks. According to different dose regimens of SVF and ADSCs, experiment rats were randomly grouped as the model control group, low-dose (LD) treatment group, middle-dose (MD) treatment group and high-dose (HD) treatment group. At 7 and 28 days post-treatment, specimens were harvested for histological and immunohistochemical analysis. We found that certain concentrations of cells (MD and HD groups) could improve the texture of photoaged skin. Changes in the epidermal cell layer were clearly observed after 7 days of treatment. The epidermal layer becomes thinner and more tender. After 28 days of treatment, the dermal tissue was thickened and the collagen content and proportion were improved. All these indicators showed no significant difference between the same dosages in the two treatment groups. Our results demonstrate that SVF may have anti-aging potential in photo-aging skin and the ADSCs play an important role in SVF. SVF maybe a potential agent for photo-anging skin and the most effective dose of SVF was 10 cells /100 µl/injection point. The proper injection interval may be 1.5 cm.
脂肪来源干细胞（ADSCs）已被用于抗光老化。但ADSCs的纯化需要扩增和培养，直接用于患者治疗存在相当大的风险。基质血管成分（SVF）是一种包含ADSCs的具有生物学和临床意义的异质性细胞群。关于SVF对光老化皮肤的抗老化作用的报道较少。本研究探讨了基质血管成分（SVF）和脂肪来源干细胞（ADSCs）注射对光老化皮肤的抗老化作用。还讨论了注射剂量与效果之间的关系。将30只健康的6周龄裸鼠随机分为对照组和实验组。实验组每周5天接受紫外线B（UVB）照射，持续8周。根据SVF和ADSCs的不同剂量方案，将实验大鼠随机分为模型对照组、低剂量（LD）治疗组、中剂量（MD）治疗组和高剂量（HD）治疗组。治疗后7天和28天，采集标本进行组织学和免疫组化分析。我们发现一定浓度的细胞（MD组和HD组）可以改善光老化皮肤的质地。治疗7天后，表皮细胞层的变化清晰可见。表皮层变薄且更细嫩。治疗28天后，真皮组织增厚，胶原蛋白含量和比例提高。两个治疗组相同剂量之间的所有这些指标均无显著差异。我们的结果表明，SVF可能对光老化皮肤具有抗老化潜力，且ADSCs在SVF中起重要作用。SVF可能是一种用于光老化皮肤的潜在药物，SVF的最有效剂量为每注射点10个细胞/100 µl。合适的注射间隔可能为1.5 cm。
