Milej Daniel, Rajaram Ajay, Suwalski Marianne, Morrison Laura B, Shoemaker Leena N, St Lawrence Keith
Lawson Health Research Institute, Imaging Program, London, Ontario, Canada.
Western University, Department of Medical Biophysics, London, Ontario, Canada.
Neurophotonics. 2022 Jul;9(3):035001. doi: 10.1117/1.NPh.9.3.035001. Epub 2022 Jul 20.
Hyperspectral near-infrared spectroscopy (hsNIRS) combined with diffuse correlation spectroscopy (DCS) provides a noninvasive approach for monitoring cerebral blood flow (CBF), the cerebral metabolic rate of oxygen ( ) and the oxidation state of cytochrome-c-oxidase (oxCCO). is calculated by combining tissue oxygen saturation ( ) with CBF, whereas oxCCO can be measured directly by hsNIRS. Although both reflect oxygen metabolism, a direct comparison has yet to be studied. We aim to investigate the relationship between and oxCCO during periods of restricted oxygen delivery and lower metabolic demand. A hybrid hsNIRS/DCS system was used to measure hemodynamic and metabolic responses in piglets exposed to cerebral ischemia and anesthetic-induced reductions in brain activity. Although a linear relationship was observed between and oxCCO during ischemia, both exhibited a nonlinear relationship with respect to CBF. In contrast, linear correlation was sufficient to characterize the relationships between and CBF and between the two metabolic markers during reduced metabolic demand. The observed relationship between and oxCCO during periods of restricted oxygen delivery and lower metabolic demand indicates that the two metabolic markers are strongly correlated.
高光谱近红外光谱（hsNIRS）与扩散相关光谱（DCS）相结合，为监测脑血流量（CBF）、脑氧代谢率（ ）和细胞色素c氧化酶（oxCCO）的氧化状态提供了一种非侵入性方法。 通过将组织氧饱和度（ ）与CBF相结合来计算，而oxCCO可通过hsNIRS直接测量。尽管两者都反映氧代谢，但尚未对它们进行直接比较研究。我们旨在研究在氧输送受限和代谢需求降低期间 与oxCCO之间的关系。使用hsNIRS/DCS混合系统测量暴露于脑缺血和麻醉诱导的脑活动降低的仔猪的血流动力学和代谢反应。尽管在缺血期间观察到 与oxCCO之间存在线性关系，但两者与CBF均呈非线性关系。相比之下，线性相关性足以表征代谢需求降低期间 与CBF之间以及两个代谢标志物之间的关系。在氧输送受限和代谢需求降低期间观察到的 与oxCCO之间的关系表明这两个代谢标志物密切相关。
