Peng Xiaobang, Ye Hang, Liu Hengzhao, Zhao Zixin, Hu Guojia, Zhao Peng
College of Urban, Rural Planning and Architectural Engineering, Shangluo University, Shangluo, China.
College of Life Sciences, Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi'an, China.
Mitochondrial DNA B Resour. 2022 Jul 18;7(7):1310-1312. doi: 10.1080/23802359.2022.2096417. eCollection 2022.
The genus is well known as the lady's slipper orchid in Orchidaceae family (Rchb.f.) Stein 1892, has important medicinal and ornamental value, which occurs in the tropical Asia. However, in recent decades, it was threatened with extinction by significantly reduced small population size. In this study, we sequenced and characterized the complete chloroplast genome of based on the Illumina Hiseq platform. The size of chloroplast genome was 156,567 bp, including a large single-copy (LSC) region of 88,243 bp, a small single-copy (SSC) region of 3652 bp, and two inverted repeat regions (IRs) of 32,336 bp. The overall GC contents of the chloroplast genome were 35.71%. A total of 122 genes were annotated, including 76 protein-coding genes, 38 transfer RNAs (tRNAs), and eight ribosomal RNAs (rRNAs). The phylogenetic analysis indicated that formed a close relationship with another species . The results will provide helpful genetic resource for further phylogenetic studies of the genus .
该属植物在兰科中作为兜兰而广为人知（Rchb.f.）Stein 1892，具有重要的药用和观赏价值，分布于热带亚洲。然而，近几十年来，由于种群规模显著减小，它面临着灭绝的威胁。在本研究中，我们基于Illumina Hiseq平台对该属植物的叶绿体全基因组进行了测序和特征分析。该属植物叶绿体基因组大小为156,567 bp，包括一个88,243 bp的大单拷贝（LSC）区域、一个3652 bp的小单拷贝（SSC）区域和两个32,336 bp的反向重复区域（IRs）。叶绿体基因组的总体GC含量为35.71%。共注释了122个基因，包括76个蛋白质编码基因、38个转运RNA（tRNA）和8个核糖体RNA（rRNA）。系统发育分析表明，该属植物与另一个物种形成了密切的关系。这些结果将为该属植物的进一步系统发育研究提供有用的遗传资源。
