Ye Hang, Liu Hengzhao, Hu Guojia, Zhao Peng
Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an, China.
Mitochondrial DNA B Resour. 2022 Jun 24;7(6):1174-1176. doi: 10.1080/23802359.2022.2088310. eCollection 2022.
Braem 1987 is a critically Endangered terrestrial orchid mainly occurred around the southern China and northern Vietnamese borders. Recently, the population size of this species has been sharply declined due to many threats such as climate change, habitat loss, and narrow distribution. In this study, the complete chloroplast genome sequence of was determined from Illumina pair-end sequencing data. The chloroplast genome was 155,886 bp in length, including a large single copy region (LSC) of 87,573 bp, a small single-copy region (SSC) of 2,831 bp, and a pair of inverted repeated regions (IRa and IRb) of 32,741 bp each. The chloroplast genome contained 121 genes corresponding to 76 protein-coding genes, 8 ribosomal RNA (rRNA) genes and 37 transfer RNA (tRNA) genes, respectively. The results of phylogenetic analysis indicated that was closely related to and in the genus based on sixteen whole chloroplast genome sequences. The results would provide a valuable resource for future genetic studies of .
布雷姆兜兰于1987年被列为极度濒危的陆生兰花，主要分布在中国南部和越南北部边境附近。近年来，由于气候变化、栖息地丧失和分布范围狭窄等诸多威胁，该物种的种群数量急剧下降。在本研究中，通过Illumina双端测序数据确定了布雷姆兜兰的完整叶绿体基因组序列。叶绿体基因组长度为155,886 bp，包括一个87,573 bp的大单拷贝区域（LSC）、一个2,831 bp的小单拷贝区域（SSC）以及两个各为32,741 bp的反向重复区域（IRa和IRb）。叶绿体基因组包含121个基因，分别对应76个蛋白质编码基因、8个核糖体RNA（rRNA）基因和37个转运RNA（tRNA）基因。系统发育分析结果表明，基于16个完整叶绿体基因组序列，布雷姆兜兰与该属中的其他两个物种关系密切。这些结果将为布雷姆兜兰未来的遗传研究提供宝贵资源。
