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Nod-样受体蛋白 3 炎性小体激活对人皮肤恶性黑素瘤 A375 细胞活性的影响机制。

Influencing Mechanism of Nod-Like Receptor Protein 3 Inflammasome Activation in A375 Cell Activity in Human Cutaneous Malignant Melanoma.

机构信息

Department of Dermatology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uyghur Autonomous Region, China.

出版信息

J Immunol Res. 2022 Jul 15;2022:7420330. doi: 10.1155/2022/7420330. eCollection 2022.

DOI:10.1155/2022/7420330
PMID:35874899
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9307359/
Abstract

This work was to investigate mechanism by which mir-22 targeting nod-like receptor protein 3 (NLRP3) inflammasome affected activity of human skin malignant melanoma (MM) A375 cells. Twenty-four mice were rolled into a control group (Group X) and an experimental group (Group Y) randomly. Without treatment in Group X, Group Y established MM model. After cell transfection, the mice were divided into group A (blank group), group B (negative group), group C (miR-22 mimics group), group D (miR-22 inhibitor group), and group E (miR-22 inhibitor+siNLRP3 group). The results were summarized as follows. The level of miR-22 mRNA in Group Y was obviously lower than that in Group X, and levels of NLRP3 and caspase-1 mRNA and NLRP3 and caspase-1 protein in Group Y were greatly higher than those in Group X ( < 0.05). The mRNA levels of miR-22 mRNA in group C were much higher in contrast to those in group A, and the mRNA levels of NLRP3 and caspase-1 were lower. The contrast results in group D and group A were the opposite, < 0.05. The levels of NLRP3 and caspase-1 proteins in group C were greatly elevated, and those in group D were decreased compared with those in group A ( < 0.05). Therefore, miR-22 may target and inhibit the activation of the NLRP3 inflammasome to reduce the activity of cutaneous malignant melanoma A375 cells.

摘要

本研究旨在探讨 miR-22 靶向核苷酸结合寡聚化结构域样受体蛋白 3（NLRP3）炎症小体影响人皮肤恶性黑素瘤（MM）A375 细胞活性的机制。将 24 只小鼠随机分为对照组（X 组）和实验组（Y 组）。X 组不做任何处理，Y 组建立 MM 模型。转染细胞后，将小鼠分为 A 组（空白组）、B 组（阴性组）、C 组（miR-22 模拟物组）、D 组（miR-22 抑制剂组）和 E 组（miR-22 抑制剂+siNLRP3 组）。结果如下。Y 组 miR-22mRNA 水平明显低于 X 组，Y 组 NLRP3 和 caspase-1mRNA 及 NLRP3 和 caspase-1 蛋白水平明显高于 X 组（ < 0.05）。与 A 组相比，C 组 miR-22mRNA 水平明显升高，NLRP3 和 caspase-1mRNA 水平明显降低（ < 0.05）。与 A 组相比，D 组 miR-22mRNA 水平明显降低，NLRP3 和 caspase-1mRNA 水平明显升高（ < 0.05）。与 A 组相比，C 组 NLRP3 和 caspase-1 蛋白水平明显升高，D 组 NLRP3 和 caspase-1 蛋白水平明显降低（ < 0.05）。综上所述，miR-22 可能通过靶向抑制 NLRP3 炎症小体的激活来降低皮肤恶性黑素瘤 A375 细胞的活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5093/9307359/88f05dfcbd77/JIR2022-7420330.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5093/9307359/fe48e5060f5f/JIR2022-7420330.001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5093/9307359/4635a1e8c6be/JIR2022-7420330.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5093/9307359/65172ec48bbf/JIR2022-7420330.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5093/9307359/c54de9d778fa/JIR2022-7420330.005.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5093/9307359/70b4e5c7dcb1/JIR2022-7420330.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5093/9307359/88f05dfcbd77/JIR2022-7420330.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5093/9307359/fe48e5060f5f/JIR2022-7420330.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5093/9307359/945da1aba2d9/JIR2022-7420330.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5093/9307359/4635a1e8c6be/JIR2022-7420330.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5093/9307359/65172ec48bbf/JIR2022-7420330.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5093/9307359/c54de9d778fa/JIR2022-7420330.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5093/9307359/4f1d973d7c0c/JIR2022-7420330.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5093/9307359/70b4e5c7dcb1/JIR2022-7420330.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5093/9307359/88f05dfcbd77/JIR2022-7420330.008.jpg

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