Li Yan, Cheng Zhenghang, Yu Fanhui, Zhang Qi, Yu Shu, Ding Fei, He Qianru
School of Biology and Basic Medical Sciences, Soochow University, Suzhou, China.
Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, China.
Front Mol Neurosci. 2022 Jul 7;15:859349. doi: 10.3389/fnmol.2022.859349. eCollection 2022.
The peripheral nervous system has remarkable regenerative capabilities. Schwann cells and fibroblasts are known to play crucial roles in these processes. In this study, we delineated the differential effects of peripheral nerve fibroblasts and cardiac fibroblasts on Schwann cells. We found that peripheral nerve fibroblasts significantly promoted Schwann cell proliferation and migration compared with cardiac fibroblasts. The cytokine array results identified 32 of 67 proteins that were considered differentially expressed in peripheral nerve fibroblasts versus cardiac fibroblasts. Among them, 25 were significantly upregulated in peripheral nerve fibroblasts compared with cardiac fibroblasts. Activin A, the protein with the greatest differential expression, clearly co-localized with fibroblasts in the sciatic never injury rat model. experiments proved that activin A secreted from nerve fibroblasts is the key factor responsible for boosting proliferation and migration of Schwann cells through ALK4, ALK5, and ALK7. Overall, these findings suggest that peripheral nerve fibroblasts and cardiac fibroblasts exhibit different patterns of cytokine secretion and activin A secreted from peripheral nerve fibroblasts can promote the proliferation and migration of Schwann cells.
外周神经系统具有显著的再生能力。已知雪旺细胞和成纤维细胞在这些过程中发挥关键作用。在本研究中，我们阐述了外周神经成纤维细胞和心脏成纤维细胞对雪旺细胞的不同影响。我们发现，与心脏成纤维细胞相比，外周神经成纤维细胞显著促进雪旺细胞的增殖和迁移。细胞因子阵列结果显示，在67种蛋白质中有32种在外周神经成纤维细胞与心脏成纤维细胞中被认为存在差异表达。其中，与心脏成纤维细胞相比，25种在外周神经成纤维细胞中显著上调。差异表达最大的蛋白质激活素A在坐骨神经损伤大鼠模型中与成纤维细胞明显共定位。实验证明，神经成纤维细胞分泌的激活素A是通过ALK4、ALK5和ALK7促进雪旺细胞增殖和迁移的关键因素。总体而言，这些发现表明外周神经成纤维细胞和心脏成纤维细胞表现出不同的细胞因子分泌模式，并且外周神经成纤维细胞分泌的激活素A可促进雪旺细胞的增殖和迁移。
