采用超高效液相色谱-四极杆飞行时间质谱联用技术对五种植物的有效成分进行系统表征，并评价其抗高尿酸血症活性。

Systematic characterisation of the effective components of five species with UPLC-Q-TOF-MS and evaluation of their anti-hyperuricaemic activity.

作者信息

Feng Shixiu, Wang Luolin, Deng Li, Zhang Min, Ma Wenyu, Liang Xiangping, Xie Huichun, Yi Bo

机构信息

Fairylake Botanical Garden, Shenzhen & Chinese Academy of Sciences, Key Laboratory of South Subtropical Plant Diversity, Shenzhen, China.

Department of gastroenterology, Shenzhen People's Hospital, (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, China.

出版信息

Nat Prod Res. 2023 May;37(10):1662-1667. doi: 10.1080/14786419.2022.2102628. Epub 2022 Jul 23.

DOI:10.1080/14786419.2022.2102628

PMID:35875993

Abstract

and were medicinal plant resource in China. In this study, the UPLC/Q-TOF-MS analysis was performed and 151 compounds were identified. PCA analysis metabolic profiles of and leaves differ significantly from the other two species, while and share similar chemical constituents. OPLS-DA highlight with an S-plot indicated that there are 14 robust known chemical markers enabling the differentiation between these five plants. leaves extracts treatment effectively reversed the body weight loss, uric acid and creatinine content, hepatic XOD activity, kidney, liver, and ankle tissues injury and inflammation induced by potassium oxonate in hyperuricemia mice. While leaves extracts treatment showed less improvement in hyperuricemia mice.

摘要

并且是中国的药用植物资源。在本研究中，进行了超高效液相色谱/四极杆飞行时间质谱分析，鉴定出151种化合物。主成分分析表明，[具体植物1]和[具体植物2]叶片的代谢谱与其他两种[具体植物3]和[具体植物4]物种有显著差异，而[具体植物1]和[具体植物2]具有相似的化学成分。正交偏最小二乘法判别分析结合S图突出显示，有14种强大的已知化学标志物能够区分这五种[具体植物]。[具体植物1]叶片提取物处理有效逆转了高尿酸血症小鼠因氧嗪酸钾诱导的体重减轻、尿酸和肌酐含量、肝脏黄嘌呤氧化酶活性、肾脏、肝脏和踝关节组织损伤及炎症。而[具体植物2]叶片提取物处理在高尿酸血症小鼠中的改善作用较小。