Zheng Dong-kun, Chen Wei-kang, Ma Shuang-cheng, Wang Jie, Luo Chen-xuan, Luo Yue-hua
Zhongguo Zhong Yao Za Zhi. 2015 May;40(9):1776-82.
This study is to establish the HPLC fingerprint and determine eight components of Callicarpa nudiflora, and provide a scientific basis for the identification and quality control. The Waters sunfire C18 column (4.6 mm x 250 mm, 5 µm) was used and the detection wavelength was 330 nm . The column temperature was 30 °C. The mobile phases were acetonitrile (A) and 0.1% formic acid (B) eluting in a gradient program at a flow rate of 1.0 mL · min(-1). The chromatographic fingerprint similarity evaluation system for tradition Chinese medicine(2012) was used for analysis. C. nudiflora from different samples were of high similarity in fingerprint and the separation of ten components was good. There was an obvious difference between other samples and C. nudiflora leaves. In quantitative analysis, the ten components showed good regression(R2 > 0 999 0) with linear ranges, and their recoveries were in the range of 96.0%-105.0%. The established qualitative and quantitative methods are highly specific, simple and accurate, which can be used for the identification and quality control of C. nudiflora.
本研究旨在建立裸花紫珠的高效液相色谱指纹图谱并测定其8种成分，为其鉴别和质量控制提供科学依据。采用Waters sunfire C18色谱柱（4.6 mm×250 mm，5 µm），检测波长为330 nm，柱温为30℃，流动相为乙腈（A）和0.1%甲酸（B），按梯度洗脱程序，流速为1.0 mL·min⁻¹。采用《中药色谱指纹图谱相似度评价系统（2012版）》进行分析。不同样品的裸花紫珠指纹图谱相似度较高，10种成分分离良好，其他样品与裸花紫珠叶有明显差异。在定量分析中，10种成分线性关系良好，回归系数（R²＞0.999 0），回收率在96.0%～105.0%之间。所建立的定性和定量方法专属性强、简便准确，可用于裸花紫珠的鉴别和质量控制。
