School of Chemistry, Sun Yat-sen University, Guangzhou 510006, China.
Biosensors (Basel). 2023 Jun 30;13(7):693. doi: 10.3390/bios13070693.
DNA-mediated nanotechnology has become a research hot spot in recent decades and is widely used in the field of biosensing analysis due to its distinctive properties of precise programmability, easy synthesis and high stability. Multi-mode analytical methods can provide sensitive, accurate and complementary analytical information by merging two or more detection techniques with higher analytical throughput and efficiency. Currently, the development of DNA-mediated multi-mode analytical methods by integrating DNA-mediated nanotechnology with multi-mode analytical methods has been proved to be an effective assay for greatly enhancing the selectivity, sensitivity and accuracy, as well as detection throughput, for complex biological analysis. In this paper, the recent progress in the preparation of typical DNA-mediated multi-mode probes is reviewed from the aspect of deoxyribozyme, aptamer, templated-DNA and G-quadruplex-mediated strategies. Then, the advances in DNA-mediated multi-mode analytical methods for biological samples are summarized in detail. Moreover, the corresponding current applications for biomarker analysis, bioimaging analysis and biological monitoring are introduced. Finally, a proper summary is given and future prospective trends are discussed, hopefully providing useful information to the readers in this research field.
DNA 介导的纳米技术在近几十年来已成为研究热点，由于其具有精确可编程性、易于合成和高稳定性等独特性质，因此被广泛应用于生物传感分析领域。多模式分析方法可以通过将两种或更多种检测技术与更高的分析通量和效率相结合，提供敏感、准确和互补的分析信息。目前，通过将 DNA 介导的纳米技术与多模式分析方法相结合来开发 DNA 介导的多模式分析方法已被证明是一种有效的方法，可极大地提高复杂生物分析的选择性、灵敏度和准确性以及检测通量。本文从脱氧核酶、适体、模板 DNA 和 G-四链体介导策略等方面综述了典型的 DNA 介导多模式探针的最新研究进展。然后，详细总结了 DNA 介导的多模式分析方法在生物样品分析中的应用进展。此外，还介绍了相应的生物标志物分析、生物成像分析和生物监测的当前应用。最后，给出了适当的总结并讨论了未来的发展趋势，希望能为该研究领域的读者提供有用的信息。
