Department of Surgery, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
Orthopedics & Sports Medicine Laboratory, Changhua Christian Hospital, Changhua, Taiwan.
Cell Transplant. 2022 Jan-Dec;31:9636897221113798. doi: 10.1177/09636897221113798.
In our daily plastic surgery practice, we have seen many chronic wounds that need new biotechnology to help and improve wound healing. Stem cells play a crucial role in regenerative medicine. Many pre-clinical researches had reported the beneficial paracrine effects of stem cell therapy for chronic wounds. Cell-friendly scaffolds may provide the protection and three-dimensional space required for adherence of stem cells, thus allowing these stem cells to proliferate and differentiate for treatment purpose. A successful scaffold may enhance the effects of stem cell therapy. In this presented series, the authors attempted to identify the most suitable scaffolds from several commercially available wound dressings that could sustain adipose-derived stromal/progenitor cells (ADSCs) survival. Therefore, we isolated ADSCs containing the green fluorescent protein (GFP) from GFP transgenic rats. The GFP (+) ADSCs and their progenies could be easily observed using a fluorescence microscope. Moreover, we analyzed the cytokines secreted in condition medium (CM) to understand the activities of ADSCs in various dressings. Our results showed that the foam dressings, hydrofiber, chitosan, and alginate plus carboxymethylcellulose were identified as the most suitable dressing materials. Higher concentrations of transforming growth factor beta (TGF-β) and vascular endothelial growth factor (VEGF) were observed 48 h after loading them with GFP (+) ADSCs. Therefore, multiple topical cell therapy using ADSCs can be performed by applying suitable dressing scaffolds without repeated needle injections to deliver the stem cells into the wound bed. Based on their fluorescence property, the GFP (+) ADSCs can also possibly be used for testing biocompatibility of medical materials in the future.
在我们的日常整形手术实践中，我们已经看到许多慢性伤口需要新的生物技术来帮助和改善伤口愈合。干细胞在再生医学中起着至关重要的作用。许多临床前研究已经报道了干细胞治疗慢性伤口的有益旁分泌作用。细胞友好型支架可为干细胞的附着提供所需的保护和三维空间，从而使这些干细胞能够增殖和分化以达到治疗目的。一个成功的支架可以增强干细胞治疗的效果。在本系列研究中，作者试图从几种市售的伤口敷料中确定最适合的支架，以维持脂肪来源的基质/祖细胞（ADSCs）的存活。因此，我们从 GFP 转基因大鼠中分离出含有绿色荧光蛋白（GFP）的 ADSCs。使用荧光显微镜可以轻松观察 GFP（+）ADSCs 及其后代。此外，我们分析了条件培养基（CM）中分泌的细胞因子，以了解 ADSCs 在各种敷料中的活性。结果表明，泡沫敷料、水纤维、壳聚糖和藻酸盐加羧甲基纤维素被鉴定为最合适的敷料材料。在加载 GFP（+）ADSCs 48 小时后，观察到转化生长因子β（TGF-β）和血管内皮生长因子（VEGF）的浓度更高。因此，可以通过应用合适的敷料支架进行多次局部细胞治疗，而无需反复用针将干细胞注射到伤口床中。基于其荧光特性，GFP（+）ADSCs 也可能在未来用于测试医用材料的生物相容性。
