Gladstone Institutes, San Francisco, California 94158, United States.
Chan Zuckerberg Biohub, San Francisco, California 94158, United States.
J Am Chem Soc. 2022 Aug 3;144(30):13663-13672. doi: 10.1021/jacs.2c04192. Epub 2022 Jul 25.
Many existing protein detection strategies depend on highly functionalized antibody reagents. A simpler and easier to produce class of detection reagent is highly desirable. We designed a single-component, recombinant, luminescent biosensor that can be expressed in laboratory strains of and . This biosensor is deployed in multiple homogeneous and immobilized assay formats to detect recombinant SARS-CoV-2 spike antigen and cultured virus. The chemiluminescent signal generated facilitates detection by an unaugmented cell phone camera. Binding-activated tandem split-enzyme (BAT) biosensors may serve as a useful template for diagnostics and reagents that detect SARS-CoV-2 antigens and other proteins of interest.
许多现有的蛋白质检测策略依赖于高度功能化的抗体试剂。因此，人们非常希望有一种更简单、更容易生产的检测试剂。我们设计了一种单组分、重组、发光生物传感器，可以在 和 的实验室菌株中表达。该生物传感器被部署在多种均相和固定化的检测方法中，以检测重组 SARS-CoV-2 刺突抗原和培养的病毒。生成的化学发光信号可通过未经增强的手机摄像头进行检测。结合激活的串联分裂酶（BAT）生物传感器可作为用于检测 SARS-CoV-2 抗原和其他感兴趣的蛋白质的诊断和试剂的有用模板。
