Ravalin Matthew, Roh Heegwang, Suryawanshi Rahul, Kumar G Renuka, Pak John, Ott Melanie, Ting Alice Y
Department of Genetics, Stanford University, Stanford, CA, USA.
Department of Chemistry, Stanford University, Stanford, CA, USA.
bioRxiv. 2022 Jun 15:2022.06.15.496006. doi: 10.1101/2022.06.15.496006.
Many existing protein detection strategies depend on highly functionalized antibody reagents. A simpler and easier to produce class of detection reagent is highly desirable. We designed a single-component, recombinant, luminescent biosensor that can be expressed in laboratory strains of and . This biosensor is deployed in multiple homogenous and immobilized assay formats to detect recombinant SARS-CoV-2 spike antigen and cultured virus. The chemiluminescent signal generated facilitates detection by an un-augmented cell phone camera. B inding A ctivated T andem split-enzyme (BAT) biosensors may serve as a useful template for diagnostics and reagents that detect SARS-CoV-2 antigens and other proteins of interest.
许多现有的蛋白质检测策略依赖于高度功能化的抗体试剂。非常需要一类更简单且易于生产的检测试剂。我们设计了一种单组分、重组、发光生物传感器，它可以在大肠杆菌和酿酒酵母的实验室菌株中表达。这种生物传感器以多种均相和固定化检测形式部署，用于检测重组严重急性呼吸综合征冠状病毒2（SARS-CoV-2）刺突抗原和培养的病毒。产生的化学发光信号便于用未增强的手机摄像头进行检测。结合激活串联分裂酶（BAT）生物传感器可作为检测SARS-CoV-2抗原和其他感兴趣蛋白质的诊断方法和试剂的有用模板。
