Biological Complexity Unit, Okinawa Institute of Science and Technology, Onna, Japan.
Department of Physics, School of Advanced Sciences, Vellore Institute of Technology, Vellore, Tamil Nadu, India.
Elife. 2022 Jul 25;11:e75884. doi: 10.7554/eLife.75884.
Replisomes are multi-protein complexes that replicate genomes with remarkable speed and accuracy. Despite their importance, their dynamics is poorly characterized, especially in vivo. In this paper, we present an approach to infer the replisome dynamics from the DNA abundance distribution measured in a growing bacterial population. Our method is sensitive enough to detect subtle variations of the replisome speed along the genome. As an application, we experimentally measured the DNA abundance distribution in populations growing at different temperatures using deep sequencing. We find that the average replisome speed increases nearly fivefold between 17 °C and 37 °C. Further, we observe wave-like variations of the replisome speed along the genome. These variations correlate with previously observed variations of the mutation rate, suggesting a common dynamical origin. Our approach has the potential to elucidate replication dynamics in mutants and in other bacterial species.
复制体是具有惊人速度和准确性复制基因组的多蛋白复合物。尽管它们很重要，但它们的动力学特性却知之甚少，尤其是在体内。在本文中，我们提出了一种从在生长中的细菌群体中测量的 DNA 丰度分布推断复制体动力学的方法。我们的方法足够灵敏，可以检测基因组上复制体速度的细微变化。作为应用，我们使用深度测序实验测量了在不同温度下生长的群体中的 DNA 丰度分布。我们发现，在 17°C 和 37°C 之间，平均复制体速度增加了近五倍。此外，我们观察到基因组上复制体速度的波动变化。这些变化与先前观察到的突变率变化相关，表明存在共同的动力学起源。我们的方法有可能阐明 突变体和其他细菌物种中的复制动力学。
