MUC1 预后标志物与膀胱癌代谢和耐药性相关：一项生物信息学研究。
A prognosis marker MUC1 correlates with metabolism and drug resistance in bladder cancer: a bioinformatics research.
机构信息
Gansu Nephro-Urological Clinical Center, Institute of Urology, Department of Urology, Key Laboratory of Urological Disease of Gansu Province, Lanzhou University Second Hospital, Lanzhou, China.
Department of Urology, Lanzhou University Second Hospital, No. 82, Cuiyingmen, Chengguan District, Lanzhou, 730030, Gansu, China.
出版信息
BMC Urol. 2022 Jul 25;22(1):114. doi: 10.1186/s12894-022-01067-8.
BACKGROUND
MUC1 is a type I transmembrane protein that plays an important role in tumor cell signal transduction. Although current studies have shown that MUC1 is upregulated in bladder cancer (BC), the specific mechanism is still unclear.
METHODS
We performed expression analysis, gene set enrichment analysis, survival analysis, immune infiltration analysis, drug sensitivity analysis, and metabolism-related gene expression analysis on TCGA-BLCA, GES31684 and GSE13507.
RESULTS
The expression of MUC1 in the tumor and lymphatic metastasis positive samples was significantly increased. Genes related to MUC1 expression were significantly enriched in immune response, ribosomes, exosomes, and energy metabolism. The results of the immune infiltration analysis showed that M1 macrophages in BC with high MUC1 expression were significantly decreased. Expression of MUC1 increases drug resistance in BC patients. In addition, MUC1 increases glycolysis, glucose uptake, and lactate production by inducing metabolic reprogramming.
CONCLUSION
MUC1 has a significant effect on the metabolism and immune cell infiltration of BC, which may be the cause of increased drug resistance, and can be used as a molecular target for the diagnosis and treatment of BC.
背景
MUC1 是一种 I 型跨膜蛋白，在肿瘤细胞信号转导中发挥重要作用。尽管目前的研究表明 MUC1 在膀胱癌（BC）中上调，但具体机制尚不清楚。
方法
我们对 TCGA-BLCA、GES31684 和 GSE13507 进行了表达分析、基因集富集分析、生存分析、免疫浸润分析、药物敏感性分析和代谢相关基因表达分析。
结果
MUC1 在肿瘤和淋巴转移阳性样本中的表达显著增加。与 MUC1 表达相关的基因在免疫反应、核糖体、外泌体和能量代谢中显著富集。免疫浸润分析结果表明，高 MUC1 表达的 BC 中 M1 巨噬细胞显著减少。MUC1 的表达增加了 BC 患者的药物耐药性。此外，MUC1 通过诱导代谢重编程增加糖酵解、葡萄糖摄取和乳酸生成。
结论
MUC1 对 BC 的代谢和免疫细胞浸润有显著影响，这可能是增加药物耐药性的原因，可作为 BC 诊断和治疗的分子靶点。
Zotero 插件