Key Laboratory of Bioactive Materials for Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.
Key Laboratory of Bioactive Materials for Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.
Mater Sci Eng C Mater Biol Appl. 2020 Apr;109:110618. doi: 10.1016/j.msec.2019.110618. Epub 2020 Jan 3.
Periodontal disease is a common complication and conventional periodontal surgery can lead to severe bleeding. Guided tissue regeneration (GTR) membranes favor periodontal regrowth, but they still have limitations, such as improper biodegradation, poor mechanical property, and no effective hemostatic property. To overcome these shortcomings, we generated unique multifunctional scaffolds. A chitosan/polycaprolactone/gelatin sandwich-like construction was fabricated by electrospinning and lyophilization. These composite scaffolds showed favorable physicochemical properties, including: appropriate porosity (<50%), pore size (about 10 μm) and mechanical stability (increasing with more PCL), good swelling and hydrophilicity. Appropriate degradation rates were approved by degradability analysis in vitro and in vivo, which resembled tissue regeneration process more closely. As shown in cell viability assay, cell attachment assay and Sirius red staining, we knew that the scaffolds had good biocompatibility, did not adversely affect cell ability for attachment, and induced high levels of collagen secretion. Experiments of blood clotting measurement in vitro showed that composite scaffolds were capable of accelerating blood clotting and could realize effective hemostasis. The results from subcutaneous implantation revealed the scaffolds had strong cell barrier effects and protection from external cell invasion. In summary, our multifunctional composite scaffolds showed optimised structure, enhanced regenerative capabilities, and serve as a basis for approaches to improve GTR designs for periodontal regeneration.
牙周病是一种常见的并发症，传统的牙周手术会导致严重出血。引导组织再生（GTR）膜有利于牙周组织的再生，但仍存在一些局限性，如生物降解不当、机械性能差和没有有效的止血性能。为了克服这些缺点，我们制备了独特的多功能支架。采用静电纺丝和冻干技术制备了壳聚糖/聚己内酯/明胶三明治结构复合材料支架。这些复合支架具有良好的物理化学性能，包括适宜的孔隙率（<50%）、孔径（约 10 μm）和机械稳定性（随着 PCL 的增加而增加）、良好的溶胀性和亲水性。体外和体内可降解性分析表明，这些复合支架具有适宜的降解率，更接近组织再生过程。细胞活力测定、细胞黏附试验和天狼猩红染色表明，支架具有良好的生物相容性，不会对细胞黏附能力产生不利影响，并且能诱导高水平的胶原分泌。体外凝血测量实验表明，复合支架能够加速血液凝固，实现有效的止血。皮下植入实验结果表明，支架具有很强的细胞屏障作用，能够防止外部细胞入侵。总之，我们的多功能复合支架具有优化的结构，增强了再生能力，为改善 GTR 设计以促进牙周组织再生提供了依据。
