Kwiecień Ewelina, Stefańska Ilona, Kizerwetter-Świda Magdalena, Chrobak-Chmiel Dorota, Didkowska Anna, Bielecki Wojciech, Olech Wanda, Anusz Krzysztof, Rzewuska Magdalena
Department of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland.
Department of Food Hygiene and Public Health Protection, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland.
Animals (Basel). 2022 Jul 18;12(14):1825. doi: 10.3390/ani12141825.
is a Gram-positive bacterium causing purulent infections in many animal species, including the European bison. However, the data about the virulence and genetic relationships of strains isolated from these wild ruminants are strongly limited. The aim of this study was to investigate the prevalence of infections in the European bison, and to evaluate the genetic diversity of isolates from these animals. In the time span of 10 years, 328 European bison from 16 different locations were examined. The standard bacteriological methods were used for isolation and identification from clinical specimens obtained from urogenital tract infections and abscesses of different locations. The presence of genes encoding known virulence factors was investigated by PCR, and the genetic diversity of strains was examined with the RAPD-PCR method. The prevalence of infections was 14.6%, and the pathogen was isolated from both female (47.9% of isolates) and male (52.1% of isolates) European bison. It should be highlighted that a considerable number of strains were isolated from the prepuce and penis infections. Therefore, the role of in the pathogenesis of should be seriously perceived. A total of 39 strains were subjected to genetic characterization. All studied strains carried the gene, while the (25.6%), (23.1%), (7.7%), (97.4%), (69.2%), (92.3%) and (15.4%) genes were present with a variable frequency among the tested strains. The virulence genotype /// was dominant. RAPD-PCR typing showed a high level of genetic diversity among European bison strains, and a total of 31 different RAPD profiles were distinguished. In a few cases, the same RAPD profile was found in strains obtained from animals living in the same area. This study provided the first data about the prevalence and genetic relationships of in the Polish population of European bison. However, further epidemiological investigations are needed to understand the routes of transmission and dissemination of the pathogen in these wild animals.
是一种革兰氏阳性细菌，可在包括欧洲野牛在内的许多动物物种中引起化脓性感染。然而，关于从这些野生反刍动物中分离出的菌株的毒力和遗传关系的数据非常有限。本研究的目的是调查欧洲野牛中感染的流行情况，并评估从这些动物中分离出的菌株的遗传多样性。在10年的时间跨度内，对来自16个不同地点的328头欧洲野牛进行了检查。使用标准细菌学方法从泌尿生殖道感染和不同部位脓肿的临床标本中分离和鉴定。通过PCR研究编码已知毒力因子的基因的存在，并使用RAPD-PCR方法检查菌株的遗传多样性。感染的流行率为14.6%，病原体从雌性（分离株的47.9%）和雄性（分离株的52.1%）欧洲野牛中均有分离。应该强调的是，相当数量的菌株是从包皮和阴茎感染中分离出来的。因此，应认真认识在发病机制中的作用。共对39株菌株进行了遗传特征分析。所有研究菌株均携带基因，而（25.6%）、（23.1%）、（7.7%）、（97.4%）、（69.2%）、（92.3%）和（15.4%）基因在测试菌株中的出现频率各不相同。毒力基因型///占主导地位。RAPD-PCR分型显示欧洲野牛菌株之间存在高度的遗传多样性，共区分出31种不同的RAPD图谱。在少数情况下，在来自同一地区动物的菌株中发现了相同的RAPD图谱。本研究提供了关于波兰欧洲野牛种群中流行情况和遗传关系的首批数据。然而，需要进一步的流行病学调查来了解病原体在这些野生动物中的传播途径和扩散情况。
