Department of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8 St, 02-786, Warsaw, Poland.
Division of Veterinary Epidemiology and Economics, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska 159C St, 02-776, Warsaw, Poland.
BMC Vet Res. 2024 Sep 6;20(1):395. doi: 10.1186/s12917-024-04262-x.
Trueperella pyogenes is an opportunistic pathogen that causes suppurative infections in various animal species, including goats. So far, only limited knowledge of phenotypic and genotypic properties of T. pyogenes isolates from goats has been gathered. In our study, we characterized the phenotypic and genotypic properties of caprine T. pyogenes isolates and established their relationship by Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR).
From 2015 to 2023, 104 T. pyogenes isolates were obtained from 1146 clinical materials. In addition, two T. pyogenes isolates were obtained from 306 swabs collected from healthy goats. A total of 51 T. pyogenes isolates were subjected to detailed phenotypic and genotypic characterization. The virulence genotype plo/nanH/nanP/fimA/fimC/luxS was predominant. All of the tested isolates showed the ability to form a biofilm but with different intensities, whereby most of them were classified as strong biofilm formers (72.5%). The high level of genetic diversity among tested caprine T. pyogenes isolates (19 different RAPD profiles) was observed. The same RAPD profiles were found for isolates obtained from one individual, as well as from other animals in the same herd, but also in various herds.
This study provided important data on the occurrence of T. pyogenes infections in goats. The assessment of virulence properties and genetic relationships of caprine T. pyogenes isolates contributed to the knowledge of the epidemiology of infections caused by this pathogen in small ruminants. Nevertheless, further investigations are warranted to clarify the routes of transmission and dissemination of the pathogen.
败血巴斯德氏菌是一种机会致病菌，可引起包括山羊在内的各种动物的化脓性感染。迄今为止，人们对来自山羊的败血巴斯德氏菌分离株的表型和基因型特性只有有限的了解。在本研究中，我们对山羊败血巴斯德氏菌分离株的表型和基因型特性进行了表征，并通过随机扩增多态性 DNA-聚合酶链反应（RAPD-PCR）建立了它们之间的关系。
2015 年至 2023 年期间，从 1146 份临床标本中获得了 104 株败血巴斯德氏菌。此外，还从 306 份健康山羊拭子中获得了 2 株败血巴斯德氏菌。共有 51 株败血巴斯德氏菌进行了详细的表型和基因型特征分析。毒力基因型 plo/nanH/nanP/fimA/fimC/luxS 占主导地位。所有测试的分离株都具有形成生物膜的能力，但强度不同，其中大多数被归类为强生物膜形成者（72.5%）。检测到的山羊败血巴斯德氏菌分离株具有很高的遗传多样性（19 种不同的 RAPD 图谱）。从同一个体获得的分离株以及同一畜群中的其他动物，甚至不同畜群中获得的分离株都具有相同的 RAPD 图谱。
本研究为山羊败血巴斯德氏菌感染的发生提供了重要数据。对山羊败血巴斯德氏菌分离株的毒力特性和遗传关系的评估有助于了解该病原体在小反刍动物感染中的流行病学。然而，需要进一步的研究来阐明该病原体的传播和扩散途径。
