Fernández-Garayzábal José F, LaFrentz Stacey, Casamayor Almudena, Abarca Eva, Mohammed Haitham H, Cuming Rosemary S, Arias Cova R, Domínguez Lucas, Vela Ana I
Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, 28040 Madrid, Spain.
Department of Animal Health, Veterinary School, Complutense University, 28040 Madrid, Spain.
Animals (Basel). 2022 Jul 18;12(14):1827. doi: 10.3390/ani12141827.
Twenty-two unidentified Gram-positive, rod-shaped organisms were recovered from the conjunctival surface of apparently healthy horses and subjected to a polyphasic taxonomic analysis. Based on cellular morphology and biochemical criteria, the isolates were tentatively assigned to the genus , although they did not match any recognized species. Comparative 16S rRNA gene sequencing studies demonstrated that all of the isolates were phylogenetically members of the genus The isolates shared 99.4 to 100% 16S rRNA gene sequence similarity among the strains and 96.5% similarity with 2385/12, which was the closest phylogenetically related species. The DNA G+C content was 58.4 mol%. The major fatty acids were C, C, C and C , while the predominant mycolic acids consisted of C, C and C. The isolates were distinguished from related species by a number of phenotypic properties. On the basis of phenotypic and phylogenetic evidence, it is proposed that the unknown isolates from horses be classified in the genus as sp. nov. The type strain of is ICM19-01138 (DSM 109759 = CCUG 73728).
从表面健康的马的结膜表面分离出22株未鉴定的革兰氏阳性杆状菌，并对其进行了多相分类分析。根据细胞形态和生化标准，这些分离株初步被归入 属，尽管它们与任何已知物种都不匹配。比较16S rRNA基因测序研究表明，所有分离株在系统发育上均属于 属。这些分离株在菌株间的16S rRNA基因序列相似性为99.4%至100%，与系统发育关系最密切的物种2385/12的相似性为96.5%。DNA G+C含量为58.4 mol%。主要脂肪酸为C、C、C 和C ，而主要分枝菌酸由C、C和C组成。这些分离株通过一些表型特征与相关物种区分开来。基于表型和系统发育证据，建议将从马分离出的未知分离株归入 属，命名为 新种。 的模式菌株为ICM19-01138（DSM 109759 = CCUG 73728）。
