Faculty of Physics and Applied Computer Science, AGH University of Science and Technology, Al. Mickiewicz 30, 30-059 Krakow, Poland.
Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, Oxfordshire, UK.
Int J Mol Sci. 2022 Jul 19;23(14):7931. doi: 10.3390/ijms23147931.
Diseases of the muscle tissue, particularly those disorders which result from the pathology of individual muscle cells, are often called myopathies. The diversity of the content of individual cells is of interest with regard to their role in both biochemical mechanisms and the structure of muscle tissue itself. These studies focus on the preliminary analysis of the differences that may occur between diseased tissues and tissues that have been recognised as a reference group. To do so, 13 samples of biopsied human muscle tissues were studied: 3 diagnosed as dystrophies, 6 as (non-dystrophic) myopathy and 4 regarded as references. From these sets of muscle biopsies, 135 completely measured muscle fibres were separated altogether, which were subjected to investigations using synchrotron radiation X-ray fluorescence (SR-XRF). Muscle fibres were analysed in terms of the composition of elements such as Br, Ca, Cl, Cr, Cu, Fe, K, Mn, P, S and Zn. The performed statistical tests indicate that all three groups (dystrophies-D; myopathies-M; references-R) show statistically significant differences in their elemental compositions, and the greatest impact, according to the multivariate discriminate analysis (MDA), comes from elements such as Ca, Cu, K, Cl and S.
肌肉组织疾病，特别是那些由单个肌肉细胞病理引起的疾病，通常被称为肌病。就其在生化机制和肌肉组织本身结构中的作用而言，单个细胞的多样性很有趣。这些研究侧重于分析疾病组织与被认为是参考组的组织之间可能存在的差异。为此，研究了 13 个人类肌肉组织活检样本：3 个被诊断为肌肉营养不良症，6 个为（非肌肉营养不良症）肌病，4 个被视为参考。从这些肌肉活检样本中总共分离出 135 根完全测量的肌肉纤维，并使用同步辐射 X 射线荧光（SR-XRF）进行了研究。对肌肉纤维的元素组成（如 Br、Ca、Cl、Cr、Cu、Fe、K、Mn、P、S 和 Zn）进行了分析。所进行的统计检验表明，所有三组（肌肉营养不良症-D；肌病-M；参考-R）在元素组成上均显示出统计学上的显著差异，根据多元判别分析（MDA），Ca、Cu、K、Cl 和 S 等元素的影响最大。
