Aquatic Crop and Resource Development, National Research Council of Canada, Halifax, NS B3H 3Z1, Canada.
Aquatic Crop and Resource Development, National Research Council of Canada, Saskatoon, SK S7N 0W9, Canada.
Int J Mol Sci. 2022 Jul 20;23(14):7985. doi: 10.3390/ijms23147985.
Catechol is a ubiquitous chemical used in the manufacturing of fragrances, pharmaceuticals and flavorants. Environmental exposure occurs in a variety of ways through industrial processes, during pyrolysis and in effluent, yet despite its prevalence, there is limited information regarding its toxicity. While the genotoxicity and gastric carcinogenicity of catechol have been described in depth, toxicological studies have potentially overlooked a number of other effects relevant to humans. Here, we have made use of a general and behavioral larval zebrafish toxicity assay to describe previously unknown catechol-based toxicological phenomena. Behavioral testing revealed catechol-induced hypoactivity at concentrations an order of magnitude lower than observable endpoints. Catechol exposure also resulted in punctate melanocytes with concomitant decreases in the expression of pigment production and regulation markers , and . Because catechol is converted into a number of toxic metabolites by tyrosinase, an enzyme found almost exclusively in melanocytes, an evaluation of the effects of catechol on these cells is critical to evaluating the safety of this chemical. This work provides insights into the toxic nature of catechol and highlights the benefits of the zebrafish larval testing platform in being able to dissect multiple aspects of toxicity with one model.
儿茶酚是一种普遍存在的化学物质，用于制造香料、药品和调味剂。环境暴露通过工业过程、热解和废水排放等多种方式发生，尽管它很普遍，但关于其毒性的信息有限。虽然儿茶酚的遗传毒性和胃致癌性已经有了深入的描述，但毒理学研究可能忽略了一些与人类相关的其他重要影响。在这里，我们利用一般的和行为性的幼鱼斑马鱼毒性测定来描述以前未知的基于儿茶酚的毒理学现象。行为测试显示，儿茶酚在浓度低一个数量级的情况下就会引起活性降低，而可观察到的终点则更高。儿茶酚暴露还导致点状黑素细胞减少，同时伴随着色素生成和调节标记物 和 的表达减少。因为儿茶酚被酪氨酸酶转化为许多有毒代谢物，而这种酶几乎只存在于黑素细胞中，所以评估儿茶酚对这些细胞的影响对于评估这种化学物质的安全性至关重要。这项工作提供了儿茶酚毒性的深入了解，并强调了斑马鱼幼鱼测试平台的优势，能够通过一个模型来剖析毒性的多个方面。
