Wang Dahui, Zhao Zhibo, Long Youhua, Fan Rong
College of Agriculture, Guizhou University, Guiyang 550025, China.
Int J Mol Sci. 2023 Sep 19;24(18):14266. doi: 10.3390/ijms241814266.
Potato Verticillium wilt, caused by , is a serious soil-borne vascular disease, which restricts the sustainable development of the potato industry, and the pathogenic mechanism of the fungus is complex. Therefore, it is of great significance to explore the important pathogenic factors of to expand the understanding of its pathology. () gene is located in the Ca signaling pathway, which is highly conserved in filamentous fungi and involved in the regulation of a variety of biological processes. In the current study, the gene in () was characterized, and its effects on the fungal pathogenicity and tolerance to fungicide stress were further studied. The results showed that the positively regulated the growth and development, conidial germination, and production of , which was necessary for the fungus to achieve pathogenicity. It also affected the formation of melanin and microsclerotia and changed the adaptability of to different environmental stresses. In addition, altered the tolerance of to different fungicides, which may be a potential target for polyoxin. Therefore, our results strongly suggest that gene is necessary for the vegetative growth, stress response, and pathogenicity of .
马铃薯黄萎病由 引起，是一种严重的土传维管束病害，制约着马铃薯产业的可持续发展，且该真菌的致病机制复杂。因此，探究 的重要致病因子对于拓展对其病理学的认识具有重要意义。（）基因位于钙信号通路中，在丝状真菌中高度保守，并参与多种生物学过程的调控。在本研究中，对（）中的 基因进行了表征，并进一步研究了其对真菌致病性和对杀菌剂胁迫耐受性的影响。结果表明， 正向调控 的生长发育、分生孢子萌发及 的产生，而这是真菌实现致病性所必需的。它还影响黑色素和微菌核的形成，并改变 对不同环境胁迫的适应性。此外， 改变了 对不同杀菌剂的耐受性，这可能是多氧霉素的一个潜在作用靶点。因此，我们的结果有力地表明， 基因对 的营养生长、应激反应和致病性是必需的。
