Transcriptomic analysis and carbohydrate metabolism-related enzyme expression across different pH values in .

INTRODUCTION

pH is one of the important factors affecting the growth and performance of microorganisms.

METHODS

We studied the pH response and plant growth-promoting (PGP) ability of using cultivation experiments and transcriptomics, and verified the expression profiles using quantitative real-time PCR.

RESULTS

pH affected the growth and PGP properties of . At pH 7, the growth rate of was rapid, whereas pH 4 and 8 inhibited mycelial growth and PGP ability, respectively. In the pot experiment, the plant height was the highest at pH 7, 56 cm, and the lowest at pH 4 and pH 5, 46.6 cm and 47 cm, respectively. Enzyme activities were highest at pH 6 to pH 7. Enzyme activities were highest at pH 6 to pH 7. Among the 1,629 differentially expressed genes (DEGs), 1,033 genes were up-regulated and 596 were down-regulated. A total of 1,623 DEGs were annotated to carbohydrate-active enzyme coding genes.

DISCUSSION

The PGP characteristics, e.g., Phosphorus solubilization ability, of were strongest at pH 7. The results provide useful information regarding the molecular mechanism of pH response.