Liang Jinpeng, Chen Yulan, Li Sisi, Liu Dongyang, Tian Hong, Xiang Quanju, Zhao Ke, Yu Xiumei, Chen Qiang, Fan Hongzhu, Zhang Lingzi, Penttinen Petri, Gu Yunfu
Department of Microbiology, College of Resources, Sichuan Agricultural University, Chengdu, China.
Liangshan Tobacco Corporation of Sichuan Province, Xichang, China.
Front Microbiol. 2024 Mar 6;15:1359830. doi: 10.3389/fmicb.2024.1359830. eCollection 2024.
pH is one of the important factors affecting the growth and performance of microorganisms.
We studied the pH response and plant growth-promoting (PGP) ability of using cultivation experiments and transcriptomics, and verified the expression profiles using quantitative real-time PCR.
pH affected the growth and PGP properties of . At pH 7, the growth rate of was rapid, whereas pH 4 and 8 inhibited mycelial growth and PGP ability, respectively. In the pot experiment, the plant height was the highest at pH 7, 56 cm, and the lowest at pH 4 and pH 5, 46.6 cm and 47 cm, respectively. Enzyme activities were highest at pH 6 to pH 7. Enzyme activities were highest at pH 6 to pH 7. Among the 1,629 differentially expressed genes (DEGs), 1,033 genes were up-regulated and 596 were down-regulated. A total of 1,623 DEGs were annotated to carbohydrate-active enzyme coding genes.
The PGP characteristics, e.g., Phosphorus solubilization ability, of were strongest at pH 7. The results provide useful information regarding the molecular mechanism of pH response.
pH是影响微生物生长和性能的重要因素之一。
我们通过培养实验和转录组学研究了[具体微生物名称未给出]的pH响应和促进植物生长（PGP）能力，并使用定量实时PCR验证了表达谱。
pH影响了[具体微生物名称未给出]的生长和PGP特性。在pH 7时，[具体微生物名称未给出]的生长速率很快，而pH 4和pH 8分别抑制了菌丝体生长和PGP能力。在盆栽实验中，株高在pH 7时最高，为56厘米，在pH 4和pH 5时最低，分别为46.6厘米和47厘米。酶活性在pH 6至pH 7时最高。在1629个差异表达基因（DEG）中，1033个基因上调，596个基因下调。共有1623个DEG被注释为碳水化合物活性酶编码基因。
[具体微生物名称未给出]的PGP特性，如解磷能力，在pH 7时最强。这些结果为[具体微生物名称未给出]的pH响应分子机制提供了有用信息。
