Luque-Alcaraz Ana G, Velazquez-Antillón Miranda, Hernández-Téllez Cynthia N, Graciano-Verdugo Abril Z, García-Flores Nadia, Iriqui-Razcón Jorge L, Silvas-García María Irene, Zazueta-Raynaud Aldo, Moreno-Vásquez María J, Hernández-Abril Pedro A
Ingeniería Biomédica, Universidad Estatal de Sonora, Hermosillo 83100, Mexico.
Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Hermosillo 83000, Mexico.
Materials (Basel). 2022 Jul 12;15(14):4838. doi: 10.3390/ma15144838.
In the present research, an orange extract (OE) was obtained and encapsulated in a zein matrix for its subsequent physicochemical characterization and evaluation of its antioxidant capacity. The OE consists of phenolic compounds and flavonoids extracted from orange peel () by ultrasound-assisted extraction (UAE). The results obtained by dynamic light scattering (DLS) and scanning electron microscopy (SEM) indicated that zein nanoparticles with orange extract (NpZOE) presented a nanometric size and spherical shape, presenting a hydrodynamic diameter of 159.26 ± 5.96 nm. Furthermore, ζ-potential evolution and Fourier transform infrared spectroscopy (FTIR) techniques were used to evaluate the interaction between zein and OE. Regarding antioxidant activity, ABTS and DPPH assays indicated no significant differences at high concentrations of orange peel extract and NpZOE; however, NpZOE was more effective at low concentrations. Although this indicates that ultrasonication as an extraction method effectively obtains the phenolic compounds present in orange peels, the nanoprecipitation method under the conditions used allowed us to obtain particles in the nanometric range with positive ζ-potential. On the other hand, the antioxidant capacity analysis indicated a high antioxidant capacity of both OE and the NpZOE. This study presents the possibility of obtaining orange extracts by ultrasound and coupling them to zein-based nanoparticulate systems to be applied as biomedical materials functionalized with antioxidant substances of pharmaceutical utility.
在本研究中，获得了一种橙子提取物（OE），并将其包裹在玉米醇溶蛋白基质中，以便随后对其进行物理化学表征和抗氧化能力评估。该OE由通过超声辅助提取（UAE）从橙皮中提取的酚类化合物和黄酮类化合物组成。通过动态光散射（DLS）和扫描电子显微镜（SEM）获得的结果表明，含有橙子提取物的玉米醇溶蛋白纳米颗粒（NpZOE）呈现纳米尺寸和球形，流体动力学直径为159.26±5.96nm。此外，还使用ζ-电位演变和傅里叶变换红外光谱（FTIR）技术来评估玉米醇溶蛋白与OE之间的相互作用。关于抗氧化活性，ABTS和DPPH测定表明，在高浓度的橙皮提取物和NpZOE下没有显著差异；然而，NpZOE在低浓度下更有效。虽然这表明超声处理作为一种提取方法有效地获得了橙皮中存在的酚类化合物，但在所使用的条件下，纳米沉淀法使我们能够获得具有正ζ-电位的纳米级颗粒。另一方面，抗氧化能力分析表明OE和NpZOE都具有较高的抗氧化能力。本研究提出了通过超声获得橙子提取物并将其与基于玉米醇溶蛋白的纳米颗粒系统偶联的可能性，以用作具有药用抗氧化物质功能化的生物医学材料。
