Freire Samanta, Grilo Teresa, Teixeira Maria Luísa, Fernandes Euclides, Poirel Laurent, Aires-de-Sousa Marta
Laboratory of Molecular Biology, Portuguese Red Cross, 1600-680 Lisboa, Portugal.
Hospital Universitário Agostinho Neto, Praia 112, Cape Verde.
Microorganisms. 2022 Jul 14;10(7):1426. doi: 10.3390/microorganisms10071426.
This study aimed to investigate, for the first time, the occurrence and characteristics of extended-spectrum β-lactamase (ESBL)- and carbapenemase-producing in Cape Verde. A total of 98 inpatients hospitalized at Hospital Universitário Agostinho Neto were screened for rectal colonization. All ESBL- and carbapenemase-producing isolates were tested for antimicrobial susceptibility and characterized by multilocus sequence typing. Mating-out assay followed by PCR-based replicon typing were performed to characterize the plasmids harboring carbapenemase encoding genes. A large proportion of patients carried ESBL- or carbapenemase-producing (56% and 6%, respectively). Among 93 ESBL-producing isolates, there were mainly (58%) and (37%). Five different ESBLs were detected, with CTX-M-15 being highly predominant (92%). Six carbapenemase-producing isolates (five and one ) were recovered, and all of the OXA-48-like type (four OXA-181, one OXA-48, and one OXA-244). The gene was located on an IncFI-type plasmid, the gene on IncFI or IncX3 plasmids, and the gene was found to be chromosomally located. The five carbapenemase-producing isolates belonged to five distinct sequence types. This study overall showed a very high prevalence of ESBL-producing , as well as the emergence of carbapenemase producers in this hospital.
本研究旨在首次调查佛得角产超广谱β-内酰胺酶（ESBL）和碳青霉烯酶细菌的发生情况及特征。对在阿戈斯蒂纽·内图大学医院住院的98名患者进行了直肠定植筛查。对所有产ESBL和碳青霉烯酶的分离株进行了药敏试验，并通过多位点序列分型进行了鉴定。进行接合外试验，随后基于PCR的复制子分型，以鉴定携带碳青霉烯酶编码基因的质粒。很大一部分患者携带产ESBL或碳青霉烯酶的细菌（分别为56%和6%）。在93株产ESBL的分离株中，主要是大肠埃希菌（58%）和肺炎克雷伯菌（37%）。检测到五种不同的ESBL，其中CTX-M-15占主导地位（92%）。分离出六株产碳青霉烯酶的分离株（五株肺炎克雷伯菌和一株大肠埃希菌），均为OXA-48样型（四株OXA-181、一株OXA-48和一株OXA-244）。blaOXA-48基因位于IncFI型质粒上，blaKPC基因位于IncFI或IncX3质粒上，blaNDM基因位于染色体上。五株产碳青霉烯酶的肺炎克雷伯菌分离株属于五个不同的序列类型。这项研究总体显示，该医院中产ESBL细菌的患病率非常高，同时也出现了产碳青霉烯酶的细菌。
