Li Menghong, Wu Gang, Wang Mingjie, Hunziker Ernst B, Liu Yuelian
Department of Oral Cell Biology, Academic Centre of Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, 1081 LA Amsterdam, The Netherlands.
Centre of Regenerative Medicine for Skeletal Tissues, Department of Clinical Research, University of Bern, 3010 Bern, Switzerland.
Nanomaterials (Basel). 2022 Jul 16;12(14):2439. doi: 10.3390/nano12142439.
Miniscrew implants (MSIs) have been widely used as temporary anchorage devices in orthodontic clinics. However, one of their major limitations is the relatively high failure rate. We hypothesize that a biomimetic calcium phosphate (BioCaP) coating layer on mini-pin implants might be able to accelerate the osseointegration, and can be a carrier for biological agents. A novel mini-pin implant to mimic the MSIs was used. BioCaP (amorphous or crystalline) coatings with or without the presence of bovine serum albumin (BSA) were applied on such implants and inserted in the metaphyseal tibia in rats. The percentage of bone to implant contact (BIC) in histomorphometric analysis was used to evaluate the osteoconductivity of such implants from six different groups (n=6 rats per group): (1) no coating no BSA group, (2) no coating BSA adsorption group, (3) amorphous BioCaP coating group, (4) amorphous BioCaP coating-incorporated BSA group, (5) crystalline BioCaP coating group, and (6) crystalline BioCaP coating-incorporated BSA group. Samples were retrieved 3 days, 1 week, 2 weeks, and 4 weeks post-surgery. The results showed that the crystalline BioCaP coating served as a drug carrier with a sustained release profile. Furthermore, the significant increase in BIC occurred at week 1 in the crystalline coating group, but at week 2 or week 4 in other groups. These findings indicate that the crystalline BioCaP coating can be a promising surface modification to facilitate early osseointegration and increase the success rate of miniscrew implants in orthodontic clinics.
微型螺钉种植体（MSIs）已在正畸临床中广泛用作临时锚固装置。然而，其主要局限性之一是相对较高的失败率。我们假设微型种植体上的仿生磷酸钙（BioCaP）涂层可能能够加速骨整合，并且可以作为生物制剂的载体。使用了一种新型的模仿MSIs的微型种植体。将含有或不含牛血清白蛋白（BSA）的BioCaP（无定形或结晶）涂层应用于此类种植体，并植入大鼠胫骨的干骺端。组织形态计量学分析中骨与种植体接触（BIC）的百分比用于评估来自六个不同组（每组n = 6只大鼠）的此类种植体的骨传导性：（1）无涂层无BSA组，（2）无涂层BSA吸附组，（3）无定形BioCaP涂层组，（4）无定形BioCaP涂层结合BSA组，（5）结晶BioCaP涂层组，和（6）结晶BioCaP涂层结合BSA组。在手术后3天、1周、2周和4周取出样本。结果表明，结晶BioCaP涂层作为具有缓释特性的药物载体。此外，结晶涂层组在第1周时BIC显著增加，而其他组在第2周或第4周时增加。这些发现表明，结晶BioCaP涂层可能是一种有前景的表面改性方法，有助于早期骨整合并提高正畸临床中微型螺钉种植体的成功率。
