Florkiewicz Wioletta, Słota Dagmara, Placek Angelika, Pluta Klaudia, Tyliszczak Bożena, Douglas Timothy E L, Sobczak-Kupiec Agnieszka
Institute of Materials Science, Faculty of Materials Science and Physics, Cracow University of Technology, 37 Jana Pawła II Av., 31-864 Krakow, Poland.
Institute of Inorganic Chemistry and Technology, Cracow University of Technology, 24 Warszawska St., 31-155 Krakow, Poland.
J Funct Biomater. 2021 Mar 30;12(2):21. doi: 10.3390/jfb12020021.
This study involves the synthesis of hydroxyapatite and describes the preparation and characterization of polymer coatings based on poly(ethylene glycol) diacrylate and poly(ethylene glycol) and modified with bovine serum albumin and hydroxyapatite. Hydroxyapatite was obtained by wet chemical synthesis and characterized by X-ray diffraction and FTIR spectroscopy, and its Ca/P molar ratio was determined (1.69 ± 0.08). The ceramic and bovine serum albumin were used in the preparation of composite materials with the polymeric matrix. The chemical composition of coatings was characterized with FTIR spectroscopy, and their morphology was recorded with SEM imaging. Moreover, the measurements of surface roughness parameters and stereometric research were performed. The prepared coatings were subjected to in vitro studies in simulated body fluid and artificial saliva. Changes in chemical composition and morphology after immersion were examined with FTIR spectroscopy and SEM imaging. Based on the conducted research, it can be stated that applied modifiers promote the biomineralization process. The roughness analysis confirmed prepared materials were characterized by the micrometer-scale topography. The materials morphology and roughness, and the morphology of the newly formed apatite deposit, were dependent on the type of the used modifier, and the artificial fluid used in in vitro studies.
本研究涉及羟基磷灰石的合成，并描述了基于聚乙二醇二丙烯酸酯和聚乙二醇、用牛血清白蛋白和羟基磷灰石改性的聚合物涂层的制备与表征。通过湿化学合成法获得羟基磷灰石，并通过X射线衍射和傅里叶变换红外光谱对其进行表征，同时测定其钙磷摩尔比（1.69±0.08）。将陶瓷和牛血清白蛋白用于制备具有聚合物基体的复合材料。用傅里叶变换红外光谱对涂层的化学成分进行表征，用扫描电子显微镜成像记录其形态。此外，还进行了表面粗糙度参数测量和立体测量研究。将制备的涂层在模拟体液和人工唾液中进行体外研究。用傅里叶变换红外光谱和扫描电子显微镜成像检查浸泡后化学成分和形态的变化。基于所进行的研究，可以说所应用的改性剂促进了生物矿化过程。粗糙度分析证实所制备的材料具有微米级的形貌特征。材料的形态和粗糙度以及新形成的磷灰石沉积物的形态取决于所用改性剂的类型以及体外研究中使用的人工流体。
