Asadi Soada, Mortezagholi Bardia, Hadizadeh Alireza, Borisov Vitaliy, Ansari Mohammad Javed, Shaker Majdi Hasan, Nishonova Azizakhon, Adelnia Hossein, Farasati Far Bahareh, Chaiyasut Chaiyavat
Department of Anlytical Chemistry, Faculty of Chemistry, Urmia University, Urmia 5756151818, Iran.
Faculty of Dentistry, Islamic Azad University Tehran Branch, Tehran 1148963537, Iran.
Pharmaceutics. 2022 Jun 27;14(7):1359. doi: 10.3390/pharmaceutics14071359.
Due to their high entrapment efficiency, anodized titanium nanotubes (TiO-NTs) are considered effective reservoirs for loading/releasing strong antibiotics whose systemic administration is associated with diverse and severe side-effects. In this study, TiO-NTs were synthesized by anodic oxidation of titanium foils, and the effects of electrolyte percentage and viscosity on their dimensions were evaluated. It was found that as the water content increased from 15 to 30%, the wall thickness, length, and inner diameter of the NTs increase from 5.9 to 15.8 nm, 1.56 to 3.21 µm, and 59 to 84 nm, respectively. Ciprofloxacin, a highly potent antibiotic, was loaded into TiO-NTs with a high encapsulation efficiency of 93%, followed by coating with different chitosan layers to achieve a sustained release profile. The prepared formulations were characterized by various techniques, such as scanning electron microscopy, differential scanning calorimetry, and contact measurement. In vitro release studies showed that the higher the chitosan layer count, the more sustained the release. Evaluation of antimicrobial activity of the formulation against two endodontic species from and revealed minimum inhibitory concentrations (MICs) of 1 µg/mL for the former and the latter. To summarize, this study demonstrated that TiO-NTs are promising reservoirs for drug loading, and that the chitosan coating provides not only a sustained release profile, but also a synergistic antibacterial effect.
由于其高包封效率，阳极氧化钛纳米管（TiO-NTs）被认为是装载/释放强效抗生素的有效载体，这些抗生素的全身给药会带来多种严重的副作用。在本研究中，通过钛箔的阳极氧化合成了TiO-NTs，并评估了电解质百分比和粘度对其尺寸的影响。结果发现，随着水含量从15%增加到30%，纳米管的壁厚、长度和内径分别从5.9增加到15.8纳米、1.56增加到3.21微米、59增加到84纳米。环丙沙星是一种高效抗生素，以93%的高包封效率载入TiO-NTs，随后用不同的壳聚糖层进行包衣以实现缓释。通过扫描电子显微镜、差示扫描量热法和接触测量等各种技术对制备的制剂进行了表征。体外释放研究表明，壳聚糖层数越高，释放越持久。对该制剂针对来自和的两种牙髓菌种的抗菌活性评估显示，前者和后者的最低抑菌浓度（MIC）均为1微克/毫升。总之，本研究表明TiO-NTs是有前景的药物装载载体，壳聚糖包衣不仅提供了缓释效果，还具有协同抗菌作用。
