Guo Shi, Song Zhengmei, Ji Ding-Kun, Reina Giacomo, Fauny Jean-Daniel, Nishina Yuta, Ménard-Moyon Cécilia, Bianco Alberto
CNRS, Immunology, Immunopathology and Therapeutic Chemistry, UPR3572, University of Strasbourg, ISIS, 67000 Strasbourg, France.
Graduate School of Natural Science and Technology, Okayama University, Tsushimanaka, Kita-ku, Okayama 700-8530, Japan.
Pharmaceutics. 2022 Jun 28;14(7):1365. doi: 10.3390/pharmaceutics14071365.
Graphene oxide (GO) is one of the most studied nanomaterials in many fields, including the biomedical field. Most of the nanomaterials developed for drug delivery and phototherapies are based on noncovalent approaches that lead to an unspecific release of physisorbed molecules in complex biological environments. Therefore, preparing covalently functionalized GO using straightforward and versatile methods is highly valuable. Phototherapies, including photothermal therapy (PTT) and photodynamic therapy (PDT), have shown great potential as effective therapeutic approaches against cancer. To overcome the limits of a single method, the combination of PTT and PDT can lead to a combined effect with a higher therapeutic efficiency. In this work, we prepare a folic acid (FA) and chlorin e6 (Ce6) double-functionalized GO for combined targeted PTT/PDT. This conjugate can penetrate rapidly into cancer cells and macrophages. A combined effect of PTT and PDT is observed, leading to a higher killing efficiency toward different types of cells involved in cancer and other diseases. Our work provides a simple protocol to prepare multifunctional platforms for the treatment of various diseases.
氧化石墨烯（GO）是许多领域中研究最多的纳米材料之一，包括生物医学领域。大多数用于药物递送和光疗的纳米材料都基于非共价方法，这会导致在复杂生物环境中物理吸附分子的非特异性释放。因此，使用直接且通用的方法制备共价功能化的GO具有很高的价值。光疗，包括光热疗法（PTT）和光动力疗法（PDT），已显示出作为对抗癌症的有效治疗方法的巨大潜力。为了克服单一方法的局限性，PTT和PDT的联合使用可产生具有更高治疗效率的联合效应。在这项工作中，我们制备了用于联合靶向PTT/PDT的叶酸（FA）和二氢卟吩e6（Ce6）双功能化的GO。这种共轭物可以迅速穿透癌细胞和巨噬细胞。观察到PTT和PDT的联合效应，对癌症和其他疾病中涉及的不同类型细胞具有更高的杀伤效率。我们的工作提供了一个简单的方案来制备用于治疗各种疾病的多功能平台。
