Xu Zhilong, Chen Jie, Li Yanan, Hu Ting, Fan Lei, Xi Juqun, Han Jie, Guo Rong
School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, 225002, China.
Institute of Translational Medicine, Department of Pharmacology, School of Medicine, Yangzhou University, Yangzhou, 225002, China.
J Colloid Interface Sci. 2022 Dec 15;628(Pt A):1033-1043. doi: 10.1016/j.jcis.2022.08.006. Epub 2022 Aug 3.
Tumor treatments based on phototherapy, such as photodynamic therapy (PDT) and photothermal therapy (PTT), are promising anticancer strategies. However, their dependence on light also poses several limitations for their application. Therefore, the establishment of a multifunctional nanotheranostic platform based on light therapy is needed to improve applicability of the technology.
We designed yolk-shell magnetic FeO@Carbon@Platinum-Chlorin e6 nanoparticles (MCPtCe6), which may be used for Magnetic resonance imaging (MRI) and synergistic catalytic-photodynamic-photothermal (catalytic-PDT-PTT) tumor therapy.
We designed to compound multiple nanozymes and solve the drawbacks of single nanozyme and give additional functionalization to nanozymes for tumor therapy. FeO has T2 weighted MRI ability. The designed yolk-shell structure can disperse FeO in the carbon shell layer, which in turn can act as a carrier for PtNPs and improve the dispersion of both FeO and Pt. Pt nanoparticles attached to the surface of N-doped carbon spheres enhanced the catalytic ability of the nanozyme to generate reactive oxygen species (ROS). The covalently linked photosensitizer chlorin e6 (Ce6) on the FeO@C@Pt (MCPt) nanozyme is essential for the therapeutic effects of PDT. MCPtCe6 can be specifically activated by the microenvironment through an enzyme-like catalytic process and extend PDT/PTT in acidic and HO-rich microenvironments. The results showed that MCPtCe6 had a high photothermal conversion efficiency (η = 28.28%), indicating its feasibility for PTT. Further cellular and animal studies have revealed that catalytic-PDT-PTT therapy can effectively inhibit tumors both in vitro and in vivo.
基于光疗的肿瘤治疗方法，如光动力疗法（PDT）和光热疗法（PTT），是很有前景的抗癌策略。然而，它们对光的依赖也给其应用带来了一些限制。因此，需要建立一个基于光疗的多功能纳米诊疗平台，以提高该技术的适用性。
我们设计了蛋黄壳型磁性FeO@碳@铂-二氢卟吩e6纳米颗粒（MCPtCe6），其可用于磁共振成像（MRI）以及协同催化-光动力-光热（催化-PDT-PTT）肿瘤治疗。
我们旨在复合多种纳米酶，解决单一纳米酶的缺点，并为纳米酶赋予额外功能以用于肿瘤治疗。FeO具有T2加权MRI能力。设计的蛋黄壳结构可将FeO分散在碳壳层中，这反过来又可作为PtNPs的载体，并改善FeO和Pt的分散性。附着在氮掺杂碳球表面的铂纳米颗粒增强了纳米酶产生活性氧（ROS）的催化能力。FeO@C@Pt（MCPt）纳米酶上共价连接的光敏剂二氢卟吩e6（Ce6）对PDT的治疗效果至关重要。MCPtCe6可通过类似酶的催化过程被微环境特异性激活，并在酸性和富H₂O₂的微环境中扩展PDT/PTT。结果表明，MCPtCe6具有高光热转换效率（η = 28.28%），表明其用于PTT的可行性。进一步的细胞和动物研究表明，催化-PDT-PTT疗法在体外和体内均可有效抑制肿瘤。
