Catalano Caterina, Abbate Loredana, Fatta Del Bosco Sergio, Motisi Antonio, Carimi Francesco, De Michele Roberto, Mercati Francesco, D'Onghia Anna Maria, Carra Angela
CNR-Istituto di Bioscienze e BioRisorse, Corso Calatafimi 414, 90129 Palermo, Italy.
CIHEAM-Centre International de Hautes Etudes Agronomiques Méditerranéennes of Bari, Via Ceglie 9, 70010 Valenzano, Italy.
Plants (Basel). 2022 Jul 8;11(14):1811. doi: 10.3390/plants11141811.
Robust protocols for the regeneration of somatic embryos in vitro are essential for the efficient use of the most modern biotechnologies. Unfortunately, in perennial trees such as , plants regenerated from juvenile tissues usually exhibit strong, undesirable juvenile characters such as thorny habit and delayed flowering and fruit production. In this work, we tested whether the cell types (nucellar and stigma/style) used to regenerate plants through somatic embryogenesis affected the transition from the juvenile to mature phase. The results show that regenerants from nucellar cells presented persistent juvenile characters, whereas plants originating from stigma/style explants transited to the mature phase more rapidly. Our observations support the hypothesis that the totipotent cells originated from different cell types are not equivalent, possibly by maintaining memory of their previously differentiated state.
用于体外体细胞胚胎再生的稳健方案对于最现代生物技术的有效利用至关重要。不幸的是，在诸如[具体树种未提及]的多年生树木中，从幼嫩组织再生的植株通常表现出强烈的、不良的幼态特征，如多刺习性以及开花和结果延迟。在这项工作中，我们测试了用于通过体细胞胚胎发生再生[具体树种未提及]植株的细胞类型（珠心和柱头/花柱）是否会影响从幼年期到成年期的转变。结果表明，来自珠心细胞的再生植株呈现出持续的幼态特征，而源自柱头/花柱外植体的植株则更快地过渡到成年期。我们的观察结果支持这样的假设，即源自不同细胞类型的全能细胞并不等同，可能是通过保持其先前分化状态的记忆。
