Galindo Fernando Shintate, Pagliari Paulo Humberto, da Silva Edson Cabral, Silva Vinicius Martins, Fernandes Guilherme Carlos, Rodrigues Willian Lima, Céu Elaine Garcia Oliveira, de Lima Bruno Horschut, Jalal Arshad, Muraoka Takashi, Buzetti Salatiér, Lavres José, Teixeira Filho Marcelo Carvalho Minhoto
Center for Nuclear Energy in Agriculture, University of São Paulo, Piracicaba 13418-900, Brazil.
Southwest Research and Outreach Center, Department of Soil, Water, and Climate, University of Minnesota, Lamberton, MN 56152, USA.
Plants (Basel). 2022 Jul 14;11(14):1847. doi: 10.3390/plants11141847.
This study was designed to investigate the effects of and sp. co-inoculation coupled with N application on soil N levels and N in plants (total N, nitrate N-NO and ammonium N-NH), photosynthetic pigments, cowpea plant biomass and grain yield. An isotopic technique was employed to evaluate N fertilizer recovery and derivation. Field trials involved two inoculations-(i) single sp. and (ii) sp. + co-inoculation-and four N fertilizer rates (0, 20, 40 and 80 kg ha). The co-inoculation of sp. + increased cowpea N uptake (an increase from 10 to 14%) and grain yield (an average increase of 8%) compared to the standard inoculation with sp. specifically derived from soil and other sources without affecting N fertilizer recovery. There is no need for the supplementation of N via mineral fertilizers when co-inoculation is performed in a cowpea crop. However, even in the case of an NPK basal fertilization, applied N rates should remain below 20 kg N ha when co-inoculation with sp. and is performed.
本研究旨在探究[具体菌种1]和[具体菌种2]联合接种并配合施氮对土壤氮水平及植物氮含量（总氮、硝态氮-NO₃⁻和铵态氮-NH₄⁺）、光合色素、豇豆植株生物量和籽粒产量的影响。采用同位素技术评估氮肥回收率和来源。田间试验涉及两种接种处理——（i）单一[具体菌种1]接种和（ii）[具体菌种1]+[具体菌种2]联合接种——以及四种氮肥施用量（0、20、40和80 kg N ha⁻¹）。与仅接种[具体菌种1]的标准接种相比，[具体菌种1]+[具体菌种2]联合接种使豇豆氮吸收量增加（从10%增至14%），籽粒产量平均提高8%，且特别源自土壤和其他来源的氮并未影响氮肥回收率。在豇豆作物中进行[具体菌种1]和[具体菌种2]联合接种时，无需通过矿物肥料补充氮。然而，即便进行氮磷钾基肥施用，在与[具体菌种1]和[具体菌种2]联合接种时，施氮量应保持在20 kg N ha⁻¹以下。
