El-Ghorab Ahmed H, Behery Fathy A, Abdelgawad Mohamed A, Alsohaimi Ibrahim Hotan, Musa Arafa, Mostafa Ehab M, Altaleb Hamud A, Althobaiti Ibrahim O, Hamza Mohamed, Elkomy Mohammed H, Hamed Ahmed A, Sayed Ahmed M, Hassan Hossam M, Aboseada Mahmoud A
Department of Chemistry, College of Science, Jouf University, Sakaka 72341, Saudi Arabia.
Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.
Plants (Basel). 2022 Jul 18;11(14):1871. doi: 10.3390/plants11141871.
L. is a medicinal plant with culinary and ethnomedical applications. Its biological and phytochemical profiles have been extensively researched. Accordingly, this study aimed to investigate the chemical composition and the antibacterial and antioxidant properties of high features, as well as to search for techniques for activity optimization. A metabolomics study of the crude extract of using liquid chromatography-high-resolution electrospray ionization mass spectrometry (LC ± HR ± ESI ± MS) was conducted. Five fractions (petroleum ether, dichloromethane, ethyl acetate, n-butanol, and aqueous) were derived from the total extract of the aerial parts. Different chromatographic methods and NMR analysis were utilized to purify and identify the isolated phenolics (high features). Moreover, the antimicrobial, antibiofilm, and antioxidant activity of phenolics were performed. Results showed that metabolomic profiling of the crude extract of aerial parts revealed the presence of a variety of phytochemicals, predominantly phenolics, resulting in the isolation and identification of seven high-feature compounds comprising two phenolic acids, rosmarinic and caffeic acids, one phenolic diterpene, 7-methoxyepirosmanol, in addition to four flavonoids, quercetin, hesperitin, hesperidin, and luteolin. On the other hand, 7-methoxyepirosmanol (OM) displayed the most antimicrobial and antioxidant potential. Such a phenolic principal activity improvement seems to be established after loading on gold nanoparticles.
L. 是一种具有烹饪和民族医学用途的药用植物。其生物学和植物化学特征已得到广泛研究。因此，本研究旨在研究其化学成分、抗菌和抗氧化特性等高级特征，并寻找活性优化技术。使用液相色谱 - 高分辨率电喷雾电离质谱（LC ± HR ± ESI ± MS）对其粗提物进行了代谢组学研究。从地上部分的总提取物中得到了五个馏分（石油醚、二氯甲烷、乙酸乙酯、正丁醇和水相）。利用不同的色谱方法和核磁共振分析对分离出的酚类物质（高级特征）进行纯化和鉴定。此外，还对酚类物质的抗菌、抗生物膜和抗氧化活性进行了检测。结果表明，对地上部分粗提物的代谢组学分析揭示了多种植物化学物质的存在，主要是酚类物质，从而分离并鉴定出七种高级特征化合物，包括两种酚酸，迷迭香酸和咖啡酸，一种酚类二萜，7 - 甲氧基表迷迭香醇，以及四种黄酮类化合物，槲皮素、橙皮素、橙皮苷和木犀草素。另一方面，7 - 甲氧基表迷迭香醇（OM）表现出最强的抗菌和抗氧化潜力。在负载到金纳米颗粒上后，这种酚类主要活性似乎得到了增强。
