Determination of nutrient content, β-carotene, and antioxidant activity of extraction using organic solution.

OBJECTIVE

The research was conducted to determine β-carotene and antioxidant activities and screening of phytochemical substances of extraction using organic solution.

MATERIALS AND METHODS

550 gm of M. oliefera leaf flour was macerated. This research was conducted by laboratory experiments using the maceration method. The extraction was performed using three kinds of solvents, which are n-hexane, ethyl acetate, and methanol; for 3 x 24 h, they were concentrated with a rotary evaporator. Then, the flavonoid, phenolic, β-carotene isolation, and antioxidant tests were conducted using the 2,2-diphenyl-1-picrylhydrazyl on each fraction (n-hexane, ethyl acetate, and methanol).

RESULTS

The results of weighing each concentrated extract from the maceration process of each fraction (n-hexane, ethyl acetate, and methanol) were 12.67, 35.67, and 49.29 gm, with the total phenolic content (1.4595 ± 0.361, 46.5489 ± 1.832, and 39.74574 ± 0.786) and total flavonoid content of each fraction (3.3056 ± 0.039, 58.6389 ± 2.051, and 48.9056 ± 0.0809), respectively. The antioxidant activity test on the crude extract from the ethyl acetate fractionation showed that the IC value was 30.309 mg/ml. The ethyl acetate fraction has a high total phenolic and flavonoid content. The results of the isolation of β-carotene from leaf flour were 0.4798 gm, or equivalent to 0.956% carotenoids.

CONCLUSIONS

Based on the results of the research, leaves are identified to have a fairly high antioxidant activity, which is 30.309 mg/ml, resulting from the potential compounds in leaves that function as inhibitors of antioxidant activity, which are the groups of phenolic and flavonoid compounds.